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| Status |
Public on Nov 28, 2022 |
| Title |
MCF7, AROE, DMSO, AR |
| Sample type |
SRA |
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| Source name |
MCF7
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| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF7
cell type: human breast cancer
chip antibody: AR, CST #5735
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| Treatment protocol |
AR expression was induced by doxycycline (800ng/ml) for 24 hours in MCF7-Tet cells. Cells were treated with DMSO or Enzalutamide (10µM) for 2 hours.
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| Growth protocol |
EMEM medium with 10% fetal bovine serum. Cells were cultured at 37C at 5% CO2.
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| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cells were fixed with 1% formaldehyde for 20 minutes. After quenching and cell lysis, chromatin was sonicated using a E220 focused-ultrasonicator (Covaris).
ChIP-seq libraries were prepared using the TruSeq ChIP Library Preparation Kit. 10 ng of DNA was used as starting material for input and ip samples.
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| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 4000 |
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| Description |
MCF7-AROE, DMSO
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| Data processing |
Sequences were aligned to the Human Reference Genome (assembly hg38, UCSC, Dec. 2013) using BWA version 0.7.17.
Enriched regions of the genome were identified by comparing the ChIP samples to input samples using the MACS2 peak caller version 2.1.
Assembly: hg38
Supplementary files format and content: Wig
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| Submission date |
Apr 06, 2022 |
| Last update date |
Nov 29, 2022 |
| Contact name |
Liewei Wang |
| E-mail(s) |
wang.liewei@mayo.edu
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| Organization name |
Mayo Clinic
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| Department |
Molecular Pharmacology and Experimental Therapeutics department
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| Street address |
200 First Street SW
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| City |
Rochester |
| State/province |
Minnesota |
| ZIP/Postal code |
55901 |
| Country |
USA |
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| Platform ID |
GPL20301 |
| Series (1) |
| GSE200300 |
Chromatin binding alteration after enzalutamide treatment |
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| Relations |
| BioSample |
SAMN27380370 |
| SRA |
SRX14758838 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM6030599_AROE-DMSO_AR-3.FCHHYK3BBXY_L8_R1_IATGTCA.PE.U12.dedup.s1.bam.s20_total_based_norm.wig.gz |
219.2 Mb |
(ftp)(http) |
WIG |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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