|
| Status |
Public on Feb 09, 2012 |
| Title |
Identification of miR-1 target genes in HNSCC, HSC3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
miR-1 transfected in HNSCC cell line (HSC3)
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HSC3
cell type: head and neck squamous cell carcinoma
miRNA: miR-1
|
| Treatment protocol |
microRNA transfection using Lipofectamine RNiMAX (Invitrogen).
|
| Growth protocol |
Normal cell culture medium RPMI 1640 or MEM.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Agilent two-color Low RNA Input Linear Amplification Kit labeling protocol.
|
| |
|
| Channel 2 |
| Source name |
control microRNA-transfected cell line (HSC3)
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HSC3
cell type: head and neck squamous cell carcinoma
miRNA: control
|
| Treatment protocol |
microRNA transfection using Lipofectamine RNiMAX (Invitrogen).
|
| Growth protocol |
Normal cell culture medium RPMI 1640 or MEM.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following the manufacturer's instructions.
|
| Label |
Cy5
|
| Label protocol |
Agilent two-color Low RNA Input Linear Amplification Kit labeling protocol.
|
| |
|
| |
| Hybridization protocol |
Agilent two-color gene expression hyb/wash protocol.
|
| Scan protocol |
Microarray slides were scanned in an Agilent Technologies G2505B Microarray Scanner at 5 micron resolution. Images were quantified using Agilent Feature Extraction software version 9.5.3.
|
| Description |
Differentially expressed genes by miR-1 transfection.
|
| Data processing |
LOWESS-normalized, background-subtracted VALUE data obtained from log10 of processed Red signal/processed Green signal. Agilent Feature Extraction software version 9.5.3 was used.
|
| |
|
| Submission date |
Oct 18, 2010 |
| Last update date |
Feb 10, 2012 |
| Contact name |
Naohiko Seki |
| E-mail(s) |
naoseki@faculty.chiba-u.jp
|
| Phone |
81-43-226-2971
|
| Fax |
81-43-227-3442
|
| Organization name |
Chiba University
|
| Department |
School of Medicine
|
| Lab |
Functional Genomics
|
| Street address |
1-8-1 Inohana,Chuo-ku
|
| City |
Chiba |
| State/province |
Chiba |
| ZIP/Postal code |
260-8670 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10332 |
| Series (1) |
| GSE24782 |
Identification of target genes of tumor suppressor microRNAs in human cancer |
|
