|
| Status |
Public on Nov 09, 2022 |
| Title |
U87_Input |
| Sample type |
SRA |
| |
|
| Source name |
U87
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: U87
cell type: Glioblastoma
chip antibody: none
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a sonicator (M220, Covaris).
Libraries were prepared for sequencing using standard MGI 2000 protocols
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
MGISEQ-2000RS |
| |
|
| Data processing |
Raw Data of ChIP-Seq was aligned to the reference genome (UCSC hg38) using BWA-MEM (v0.7.17)
Peaks were identified using MACS2 (v2.2.7)
Assembly: hg38
Supplementary files format and content: peak text files
Supplementary files format and content: bigWig, narrowPeak (except for Input sample)
|
| |
|
| Submission date |
May 08, 2022 |
| Last update date |
Nov 09, 2022 |
| Contact name |
Gen Li |
| E-mail(s) |
ligen925@yeah.net
|
| Organization name |
Children's Hospital of Soochow University
|
| Street address |
No. 92 Zhongnan Street
|
| City |
Suzhou |
| State/province |
Jiangsu |
| ZIP/Postal code |
215025 |
| Country |
China |
| |
|
| Platform ID |
GPL30209 |
| Series (1) |
| GSE202484 |
BRD4 inhibitor GNE987 exerts anti-cancer effects by targeting C-Myc and S100A16 in Glioblastoma [ChIP-seq] |
|
| Relations |
| BioSample |
SAMN28156684 |
| SRA |
SRX15206724 |
