|
| Status |
Public on Nov 13, 2010 |
| Title |
PCNSlymphoma_rep4 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
PCNSL tumor sample
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Brain
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Fresh-frozen samples were obtained from 12 patients with primary central nervous system diffuse large B cell lymphoma and 13 peripheral blood from healthy men.
|
| Label |
Cy5
|
| Label protocol |
1.5 ug of test DNA and reference DNAs were digested with Alu I and Rsa I (Promega, Madison, WI) and purified with the QIAprep Spin Miniprep kit (QIAGEN, Germantown,MD). Test sample DNA and reference DNA were labeled by random priming with either Cy3-dUTP or Cy5-dUTP using the Agilent Genomic DNA Labeling Kit PLUS.
|
| |
|
| Channel 2 |
| Source name |
Reference DNA
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Human reference DNA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Fresh-frozen samples were obtained from 12 patients with primary central nervous system diffuse large B cell lymphoma and 13 peripheral blood from healthy men.
|
| Label |
Cy3
|
| Label protocol |
1.5 ug of test DNA and reference DNAs were digested with Alu I and Rsa I (Promega, Madison, WI) and purified with the QIAprep Spin Miniprep kit (QIAGEN, Germantown,MD). Test sample DNA and reference DNA were labeled by random priming with either Cy3-dUTP or Cy5-dUTP using the Agilent Genomic DNA Labeling Kit PLUS.
|
| |
|
| |
| Hybridization protocol |
After probe denaturation and preannealing with Cot-1 DNA, hybridization was performed at 65oC with rotation for 40 hours.
|
| Scan protocol |
Scanned on an Agilent DNA microarray scanner.
|
| Description |
CGH
|
| Data processing |
Data were obtained using Agilent Feature Extraction Software 9 and analyzed with Agilent CGH Analytics 4.0 Software.
|
| |
|
| Submission date |
Nov 12, 2010 |
| Last update date |
Nov 12, 2010 |
| Contact name |
Chang Ohk Sung |
| Organization name |
Asan Medical Center
|
| Department |
Pathology
|
| Street address |
Song-Pa gu,Pung-nap dong
|
| City |
Seoul |
| ZIP/Postal code |
05505 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL8841 |
| Series (2) |
| GSE25298 |
Genomic aberrations in primary central nervous system lymphoma (PCNSL) |
| GSE25299 |
Genomic profiling combined with gene expression profiling in primary central nervous system lymphoma |
|
