|
| Status |
Public on Nov 17, 2010 |
| Title |
si FoxA1 and estrogen replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
MCF-7 cell line treated with FoxA1 siRNA and estrogen
|
| Organism |
Homo sapiens |
| Characteristics |
cell: MCF-7
treatment: FoxA1 siRNA and estrogen
|
| Treatment protocol |
Estrogen was added at a final concentration of 100 nM.
|
| Growth protocol |
MCF-7 human cell lines were grown as described previously (Neve et al. 2006, Cancer Cell 10:515–527).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. A DNase I digestion was included. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
The bead-level data were preprocessed using BASH (Cairns et al. (2008) Bioinformatics 24(24):2921-2), a function from the beadarray package (Dunning et al (2007) Bioinformatics 23(16):2183-4) in Bioconductor, and also log base 2 transformed and quantile normalised using the beadarray package. Detection p-val not provided.
|
| |
|
| Submission date |
Nov 12, 2010 |
| Last update date |
Nov 17, 2010 |
| Contact name |
Roslin Russell |
| E-mail(s) |
roslin.russell@cruk.cam.ac.uk
|
| Phone |
01223 769770
|
| Organization name |
Cambridge Research Institute, Cancer Research UK
|
| Street address |
Robinson Way
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0RE |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE25315 |
FoxA1 is a critical determinant of Estrogen Receptor function and endocrine response (part II) |
| GSE25316 |
FoxA1 is a critical determinant of Estrogen Receptor function and endocrine response |
|
