|
| Status |
Public on Jan 03, 2011 |
| Title |
shMYB Day 5 of diff, rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
shMYB lentivirus transduced CD34 cells at day 5 of differentiation, replicate 3
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: CD34-derived erythroid progenitors
treatment: MYB shRNA Knockdown
|
| Treatment protocol |
CD34 cells were transduced with control or MYB shRNAs using lentiviral transduction prior to differentiation.
|
| Growth protocol |
CD34 cells were grown in a two-phase culture as has been described in detail previously (Sankaran et al., Science, 2008) and is described in the accompanying manuscript.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the miRNAeasy Mini Kit from Qiagen and following the standard protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-2 ug total RNA (3' IVT Express Kit manual, 2008, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on to the GeneChip array. GeneChips were washed and stained in the Affymetrix Fluidics Station. Details are available at: http://jura.wi.mit.edu/genomecorewiki/index.php/Main_Page.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner. Details are available at: http://jura.wi.mit.edu/genomecorewiki/index.php/Main_Page.
|
| Description |
Expression data from total RNA of CD34-derived erythroid progenitors
|
| Data processing |
Array data were normalized with the Robust Multichip Averaging (RMA) protocol using the affy package in BioConductor.
|
| |
|
| Submission date |
Nov 29, 2010 |
| Last update date |
Apr 02, 2012 |
| Contact name |
Vijay G. Sankaran |
| Organization name |
Children's Hospital Boston
|
| Department |
Department of Medicine
|
| Street address |
300 Longwood Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL8019 |
| Series (1) |
| GSE25678 |
Expression Profiling of Erythroid Progenitors After MYB shRNA Knockdown |
|
| Relations |
| Reanalyzed by |
GSM908108 |
