|
| Status |
Public on Nov 17, 2022 |
| Title |
DLPFC expression from sample 9-129 |
| Sample type |
RNA |
| |
|
| Source name |
postmortem dorsolateral prefrontal cortex tissue
|
| Organism |
Homo sapiens |
| Characteristics |
sample: 9-129
diagnosis: CTL
dx: control
age: 52.7
Sex: M
ph: 6.61
pmi: 43
rin: 7.7
rin²: 59.29
suicide: N
type of death: Natural
c1: -0.00998167
c2: -0.016579
c3: 0.00759598
c4: 0.0111196
sv1: -0.0280203759681071
adhd prs: -0.00255035297
bd prs: -0.00548844201
cdg prs: 0.00902821716
mdd-2018 prs: -0.00101306642
mdd-2019 prs: -0.00406926414
scz prs: -0.00244296648
ea prs: 2.93712574e-05
t2d prs: -0.00310131771
tissue: postmortem dorsolateral prefrontal cortex (DLPFC)
|
| Treatment protocol |
not applicable
|
| Growth protocol |
Subjects were refrigerated within 5 hours of being found. Brodmann area 9 (BA 9) was taken from the lateral surface of the frontal lobe from an area comprising the middle frontal superior gyrus to the inferior frontal sulcus of the left hemisphere. After removal at autopsy, brain tissue was rapidly processed and frozen at -70°C using a standardized procedure that minimizes autolytic effects.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from ∼100 mg frozen gray matter using 1.0 ml TRIzol reagent (Life Technologies). After homogenization and phase separation, an equal volume of 70% ethanol was added to the aqueous phase. RNA isolation was performed using RNeasy minikits (Qiagen), and all samples were treated with DNase by column digestion. RNA quantity and quality were analyzed by spectrophotometry with NanoDrop (Thermo Fischer Science) and by determination of RNA integrity numbers (RINs) using an Agilent 2100 Bioanalyzer (Agilent Technologies). All samples with RIN ⩾ 6.00 were used for further analysis with Affymetrix Human Exon 1.0 ST v2 arrays (Affymetrix).
|
| Label |
biotin
|
| Label protocol |
Labeling was performed by the Australian Genome Research Facility (AGRF; Melbourne, Australia), which performed cRNA labeling of the samples with biotin using the Affymetrix Synthesis and Labeling Kit.
|
| |
|
| Hybridization protocol |
Hybridization was conducted by the Australian Genome Research Facility (AGRF; Melbourne, Australia), which prepared samples for hybridization with a standard probe cocktail and hybridized them overnight in an Affymetrix Human Exon 1.0 ST v2 Array.
|
| Scan protocol |
Scanning of the arrays was carried out by the Australian Genome Research Facility (AGRF; Melbourne, Australia) according to the protocol provided by Affymetrix.
|
| Description |
DLPFC expression control sample
|
| Data processing |
Data were processed using the oligo package version 1.50.0 in R 3.6.1. Oligo’s Robust Multichip Average (RMA) algorithm was used for background adjustment, quantile normalisation and summarization (using median-polish) at the probeset level. Probesets that did not contain start or stop information, were labelled as controls in the current NetAffx annotation for Affymetrix’s HuEx 1.0 ST v2 array, were cross-hybridized (number_cross_hyb_probes ≥ 1), or were located on X, Y and M chromosomes were removed from the data set. The SVA package version 3.34.0 in R was used for batch correction of known and hidden batches.
HuEx-1_0-st-v2.r2.pgf
|
| |
|
| Submission date |
Jul 17, 2022 |
| Last update date |
Nov 17, 2022 |
| Contact name |
Karolina Worf |
| E-mail(s) |
karolina.worf@gmail.com
|
| Organization name |
Helmholtz Zentrum Muenchen
|
| Department |
Computational Health
|
| Street address |
Ingolstaedter Landstr. 1
|
| City |
Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL5188 |
| Series (1) |
| GSE208338 |
Expression data from postmortem human dorsolateral prefrontal cortex - psychiatric disorders & healthy controls |
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