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GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 10, 2017 |
| Title |
Regenerating_MM11 |
| Sample type |
RNA |
| |
|
| Source name |
Laser captured spinal cord neurons
|
| Organism |
Rattus norvegicus |
| Characteristics |
Fischer 344, adult female
|
| Biomaterial provider |
Lawrence Moon
|
| Treatment protocol |
Cut 10um transverse cryosections of unfixed spinal cord, dehydrate, laser capture
|
| Growth protocol |
n/a
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PicoPure (version C), Arcturus
|
| Label |
Biotin
|
| Label protocol |
Three round Eberwine amplification, proprietary modified Message Amp using BioArray High Yield labeling kit (Enzo) in last round.
|
| |
|
| Hybridization protocol |
Standard Affymetrix procedure for fragmentation and hybridization
|
| Scan protocol |
Standard GCOS scanning, GeneChip Scanner 3000
|
| Description |
Identification of genes associated with axotomy or regeneration in the CNS.
|
| Data processing |
GCOS, scaled to 200
|
| |
|
| Submission date |
Jul 12, 2005 |
| Last update date |
Dec 10, 2017 |
| Contact name |
Lawrence DF Moon |
| E-mail(s) |
lawrence.moon@kcl.ac.uk
|
| Organization name |
King's College London
|
| Department |
Wolfson Centre for Age-Related Diseases
|
| Lab |
Moon team
|
| Street address |
16 - 18 Newcomen Street
|
| City |
London |
| ZIP/Postal code |
SE11UL |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE2925 |
Identification of genes expressed by spinal cord neurons after axotomy and during axon regeneration |
|
| Data table header descriptions |
| ID_REF |
|
| VALUE |
TGT set to 200, NF=1.0000, SF Gene=All, Standard GCOS algorithm for Signal estimation |
| ABS_CALL |
Tau=0.015 |
| DETECTION P-VALUE |
Standard GCOS algorithm |
| Data table |
| ID_REF |
VALUE |
ABS_CALL |
DETECTION P-VALUE |
| AFFX-BioB-5_at |
23 |
P |
0.009337 |
| AFFX-BioB-M_at |
28.2 |
P |
0.003212 |
| AFFX-BioB-3_at |
28.7 |
M |
0.062929 |
| AFFX-BioC-5_at |
147.6 |
P |
0.00011 |
| AFFX-BioC-3_at |
128.6 |
P |
0.000147 |
| AFFX-BioDn-5_at |
235.8 |
P |
0.000044 |
| AFFX-BioDn-3_at |
678.3 |
P |
0.000044 |
| AFFX-CreX-5_at |
1186.8 |
P |
0.000052 |
| AFFX-CreX-3_at |
1409 |
P |
0.000044 |
| AFFX-DapX-5_at |
98.7 |
P |
0.000225 |
| AFFX-DapX-M_at |
168.3 |
P |
0.000258 |
| AFFX-DapX-3_at |
224.2 |
P |
0.000169 |
| AFFX-LysX-5_at |
117.2 |
P |
0.00011 |
| AFFX-LysX-M_at |
147.8 |
P |
0.00006 |
| AFFX-LysX-3_at |
165.8 |
P |
0.00006 |
| AFFX-PheX-5_at |
151.7 |
P |
0.00007 |
| AFFX-PheX-M_at |
209.2 |
P |
0.000044 |
| AFFX-PheX-3_at |
157.1 |
P |
0.000052 |
| AFFX-ThrX-5_at |
175.9 |
P |
0.00006 |
| AFFX-ThrX-M_at |
156.4 |
P |
0.000052 |
Total number of rows: 31099
Table truncated, full table size 819 Kbytes.
| Supplementary file |
Size |
Download |
File type/resource |
| GSM63822.CEL.gz |
4.8 Mb |
(ftp)(http) |
CEL |
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