|
| Status |
Public on Dec 10, 2017 |
| Title |
Non-regenerating_MOON265 |
| Sample type |
RNA |
| |
|
| Source name |
Laser captured spinal cord neurons
|
| Organism |
Rattus norvegicus |
| Characteristics |
Adult female Fischer 344
|
| Biomaterial provider |
Lawrence Moon
|
| Treatment protocol |
Cut 10um transverse cryosections of unfixed spinal cord, dehydrate, laser capture
|
| Growth protocol |
n/a
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PicoPure (version C), Arcturus
|
| Label |
Biotin
|
| Label protocol |
Three round Eberwine amplification, proprietary modified Message Amp using BioArray High Yield labeling kit (Enzo) in last round.
|
| |
|
| Hybridization protocol |
Standard Affymetrix procedure for fragmentation and hybridization
|
| Scan protocol |
Standard GCOS scanning, GeneChip Scanner 3000
|
| Description |
Identification of genes associated with axotomy or regeneration in the CNS.
|
| Data processing |
GCOS, scaled to 200
|
| |
|
| Submission date |
Jul 12, 2005 |
| Last update date |
Dec 10, 2017 |
| Contact name |
Lawrence DF Moon |
| E-mail(s) |
lawrence.moon@kcl.ac.uk
|
| Organization name |
King's College London
|
| Department |
Wolfson Centre for Age-Related Diseases
|
| Lab |
Moon team
|
| Street address |
16 - 18 Newcomen Street
|
| City |
London |
| ZIP/Postal code |
SE11UL |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE2925 |
Identification of genes expressed by spinal cord neurons after axotomy and during axon regeneration |
|
