|
Status |
Public on Jul 28, 2023 |
Title |
platelts-ALS-nonmat-16 [16_PLT] |
Sample type |
other |
|
|
Source name |
Platelets isolated from fresh EDTA blood of a fALS patient with non-maternal inheritance
|
Organism |
Homo sapiens |
Characteristics |
mat vs non-mat: non-maternal disease state: ALS age: 73 gender: m mutation: o.B. cell type: platelets
|
Extracted molecule |
other |
Extraction protocol |
QiaAmp Blood Kit for DNA isolation; REPLI-g mitochondrial DNA kit (Qiagen, Hilden, Germany) for amplification of mtDNA; Purification using Agencourt® AMPure® XP magnetic beads (QIAGEN Supplementary Protocol, REPLI-g mitochondrial DNA kit)
|
Label |
biotin
|
Label protocol |
GeneChip® CustomSeq® Resquencing Array Protocol Version 2.1
|
|
|
Hybridization protocol |
GeneChip® CustomSeq® Resquencing Array Protocol Version 2.1 ; 270ng of fragmented mtDNA were hybridized to GeneChip™ Human Mitochondrial Resequencing Arrays 2.0 (MitoChip v2.0, Zhou et al., 2006 ).
|
Scan protocol |
GeneChip® CustomSeq® Resquencing Array Protocol Version 2.1; The Arrays were washed using Affymetrix fluidics station FS450, and scanned using the Gene Chip Scanner 3000 7G.
|
Description |
isolation of platelets by differential centrifugation and subsequent clean up of the sample by depletion of CD45 positive cells using magnetic beads (MACS miltenyi)
|
Data processing |
GSEQ Version 4.1.1
|
|
|
Submission date |
Aug 15, 2022 |
Last update date |
Jul 28, 2023 |
Contact name |
Karin Danzer |
E-mail(s) |
karin.danzer@uni-ulm.de
|
Organization name |
DZNE
|
Street address |
Albert-Einstein-Allee 11
|
City |
Ulm |
ZIP/Postal code |
89069 |
Country |
Germany |
|
|
Platform ID |
GPL10983 |
Series (1) |
GSE211250 |
Mitochondrial DNA sequences of familial (maternal vs. non-maternal) ALS cases in whole blood, platelets and white blood cells |
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