|
| Status |
Public on Jul 28, 2023 |
| Title |
platelets-ALS-nonmat-25 [25_PLT] |
| Sample type |
other |
| |
|
| Source name |
Platelets isolated from fresh EDTA blood of a fALS patient with non-maternal inheritance
|
| Organism |
Homo sapiens |
| Characteristics |
mat vs non-mat: non-maternal
disease state: ALS
age: 67
gender: m
mutation: o.B.
cell type: platelets
|
| Extracted molecule |
other |
| Extraction protocol |
QiaAmp Blood Kit for DNA isolation; REPLI-g mitochondrial DNA kit (Qiagen, Hilden, Germany) for amplification of mtDNA; Purification using Agencourt® AMPure® XP magnetic beads (QIAGEN Supplementary Protocol, REPLI-g mitochondrial DNA kit)
|
| Label |
biotin
|
| Label protocol |
GeneChip® CustomSeq® Resquencing Array Protocol Version 2.1
|
| |
|
| Hybridization protocol |
GeneChip® CustomSeq® Resquencing Array Protocol Version 2.1 ; 270ng of fragmented mtDNA were hybridized to GeneChip™ Human Mitochondrial Resequencing Arrays 2.0 (MitoChip v2.0, Zhou et al., 2006 ).
|
| Scan protocol |
GeneChip® CustomSeq® Resquencing Array Protocol Version 2.1; The Arrays were washed using Affymetrix fluidics station FS450, and scanned using the Gene Chip Scanner 3000 7G.
|
| Description |
isolation of platelets by differential centrifugation and subsequent clean up of the sample by depletion of CD45 positive cells using magnetic beads (MACS miltenyi)
|
| Data processing |
GSEQ Version 4.1.1
|
| |
|
| Submission date |
Aug 15, 2022 |
| Last update date |
Jul 28, 2023 |
| Contact name |
Karin Danzer |
| E-mail(s) |
karin.danzer@uni-ulm.de
|
| Organization name |
DZNE
|
| Street address |
Albert-Einstein-Allee 11
|
| City |
Ulm |
| ZIP/Postal code |
89069 |
| Country |
Germany |
| |
|
| Platform ID |
GPL10983 |
| Series (1) |
| GSE211250 |
Mitochondrial DNA sequences of familial (maternal vs. non-maternal) ALS cases in whole blood, platelets and white blood cells |
|
