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Sample GSM669613 Query DataSets for GSM669613
Status Public on Mar 31, 2011
Title MeDIP-Seq analysis of ganglionic eminence neural stem and progenitor cells from HuFNSC02 (HS2781)
Sample type SRA
 
Source name HS2781-1
Organism Homo sapiens
Characteristics submitted sample id: JOC78-DNA
donor_id: HuFNSC02
Sex: female
body site: Brain
histological type: Ganglionic eminence neurosphere culture
is tumor: No
biomaterial_type: primary cell culture
cell type: ganglionic eminence neural stem and progenitor cells
Extracted molecule genomic DNA
Extraction protocol library construction protocol: Refer to document 'MeDIP-seq library construction protocol v2' from the Costello Lab at the Roadmap Epigenomics Project site, Experimental Protocols page (URL: http://www.roadmapepigenomics.org/protocols/type/experimental/)
 
Library strategy MeDIP-Seq
Library source genomic
Library selection 5-methylcytidine antibody
Instrument model Illumina Genome Analyzer IIx
 
Description design description: MeDIP-Seq analysis of ganglionic eminence neural stem and progenitor cells from HuFNSC02 (HS2781) using Illumina Genome Analyzer IIx
library name: HS2781
EXPERIMENT_TYPE: DNA Methylation
EXTRACTION_PROTOCOL: Refer to document 'MeDIP-seq library construction protocol v2' by Costello Lab at the Roadmap Epigenomics Project site, Experimental Protocols page (URL: http://www.roadmapepigenomics.org/protocols/type/experimental/)
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Diagenode Bioruptor
EXTRACTION_PROTOCOL_SONICATION_CYCLES: 25 min
MeDIP_PROTOCOL: Refer to document 'MeDIP-seq library construction protocol v2' by Costello Lab at the Roadmap Epigenomics Project site, Experimental Protocols page (URL: http://www.roadmapepigenomics.org/protocols/type/experimental/)
MeDIP_PROTOCOL_DNA_AMOUNT: 2 ug
MeDIP_PROTOCOL_BEAD_TYPE: Protein A/G Beads
MeDIP_PROTOCOL_BEAD_AMOUNT: 100 ul
MeDIP_PROTOCOL_ANTIBODY_AMOUNT: 1 ug
MeDIP_ANTIBODY: Methylcytidine
MeDIP_ANTIBODY_PROVIDER: Eurogentec
MeDIP_ANTIBODY_CATALOG: BI-MECY-0100
MeDIP_ANTIBODY_LOT: 80808
****************
For data usage terms and conditions, please refer to:
http://www.drugabuse.gov/funding/funding-opportunities/nih-common-fund/epigenomics-data-access-policies
****************
Data processing **********************************************************************

ANALYSIS FILE NAME: GSM669613_UCSF-UBC.Neurosphere_Cultured_Cells_Ganglionic_Eminence_Derived.MeDIP-Seq.HuFNSC02.bed
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: HS2781-1.hg19.level.1.release.3
ANALYSIS TITLE: Mapping of Neurosphere Cultured Cells Ganglionic Eminence Derived MeDIP-Seq Data
ANALYSIS DESCRIPTION: Illumina reads produced by MeDIP-Seq on Neurosphere Cultured Cells Ganglionic Eminence Derived, Donor HuFNSC02 were mapped to the human genome using Pash.
ANALYSIS TYPE: REFERENCE_ALIGNMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.6334
DATA_ANALYSIS_LEVEL: 1
EXPERIMENT_TYPE: MeDIP-Seq
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: Pash
SOFTWARE_VERSION: 3.0
MAXIMUM_ALIGNMENT_LENGTH: Read length
MISMATCHES_ALLOWED: 10% of read length
ALIGNMENTS_ALLOWED: 1
TREATMENT_OF_MULTIPLE_ALIGNMENTS: If a read maps to more than 1 position it is removed from consideration.
TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: None
ALIGNMENT_POSTPROCESSING: None
RELEASE_NUMBER: Human Epigenome Atlas 3


QUALITY SCORES:
NUMBER_OF_MEDIP-SEQ_EXPERIMENTS_SCORED_IN_THIS_RELEASE: 10
FINDPEAKS_SCORE: 0.2364
FINDPEAKS_PERCENTILE: 80
HOTSPOT_SCORE: 0.3672
HOTSPOT_PERCENTILE: 80
IROC_SCORE: 0.9484
IROC_PERCENTILE: 80
POISSON_SCORE: 0.4795
POISSON_PERCENTILE: 80

**********************************************************************

ANALYSIS FILE NAME: GSM669613_UCSF-UBC.Neurosphere_Cultured_Cells_Ganglionic_Eminence_Derived.MeDIP-Seq.HuFNSC02.wig
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: HS2781-1.hg19.level.2.release.3
ANALYSIS TITLE: Raw Signal Density Graphs of Neurosphere Cultured Cells Ganglionic Eminence Derived MeDIP-Seq Data
ANALYSIS DESCRIPTION: Illumina MeDIP-Seq read mappings from Neurosphere Cultured Cells Ganglionic Eminence Derived, Donor HuFNSC02 were processed into density graphs of raw signal representing the aligned read density.
ANALYSIS TYPE: ABUNDANCE_MEASUREMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.6344
DATA_ANALYSIS_LEVEL: 2
EXPERIMENT_TYPE: MeDIP-Seq
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: In house programs and scripts
SOFTWARE_VERSION: NA
READ_EXTENSION: 200bp
GENOMIC_WINDOW: 20bp
TREATMENT_OF_REGIONS_PRONE_TO_MULTIPLE_ALIGNMENTS: None
RELEASE_NUMBER: Human Epigenome Atlas 3
BROWSER_TRACK_NAME: NGED MeDIP 02
BROWSER_TRACK_DESCRIPTION: UCSF-UBC-USC Neurosphere Cultured Cells Ganglionic Eminence Derived MeDIP-Seq Donor HuFNSC02 Library HS2781-1 EA Release 3


QUALITY SCORES:
NUMBER_OF_MEDIP-SEQ_EXPERIMENTS_SCORED_IN_THIS_RELEASE: 10
FINDPEAKS_SCORE: 0.2364
FINDPEAKS_PERCENTILE: 80
HOTSPOT_SCORE: 0.3672
HOTSPOT_PERCENTILE: 80
IROC_SCORE: 0.9484
IROC_PERCENTILE: 80
POISSON_SCORE: 0.4795
POISSON_PERCENTILE: 80

**********************************************************************

 
Submission date Feb 07, 2011
Last update date May 15, 2019
Contact name UCSF-UBC CENTER
Organization name UCSF-UBC
Street address UCSF-UBC
City San Francisco
State/province CA
ZIP/Postal code 94143
Country USA
 
Platform ID GPL10999
Series (1)
GSE16368 UCSF-UBC Human Reference Epigenome Mapping Project
Relations
Reanalyzed by GSE69894
Named Annotation GSM669613_UCSF-UBC.Neurosphere_Cultured_Cells_Ganglionic_Eminence_Derived.MeDIP-Seq.HuFNSC02.wig.gz
SRA SRX1157932
BioSample SAMN03416705

Supplementary file Size Download File type/resource
GSM669613_UCSF-UBC.Neurosphere_Cultured_Cells_Ganglionic_Eminence_Derived.MeDIP-Seq.HuFNSC02.bed.gz 1.1 Gb (ftp)(http) BED
GSM669613_UCSF-UBC.Neurosphere_Cultured_Cells_Ganglionic_Eminence_Derived.MeDIP-Seq.HuFNSC02.wig.gz 67.4 Mb (ftp)(http) WIG
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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