GEO help: Mouse over screen elements for information.
Status
Public on Sep 26, 2005
Title
Control replicate 4
Sample type
RNA
Source name
Mouse heart
Organism
Mus musculus Characteristics
Control, untreated
Extracted molecule
total RNA
Extraction protocol
Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.
Label
biotin
Label protocol
Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Control, untreated
Data processing
Robust Multichip Analysis (RMA)
Submission date
Aug 05, 2005
Last update date
Sep 26, 2005
Contact name
Norman Buroker
E-mail(s)
Norman.Buroker@seattlechildrens.org Organization name
Seattle Children's Hospital
Lab
Portman
Street address
4800 Sand Point Way N.E.
City
Seattle
State/province
WA
ZIP/Postal code
98105
Country
USA
Platform ID
GPL1261 Series (1)
GSE3067 Resistance to thyroid hormone (RTH) and PPARa activation
Data table header descriptions ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
306.9
P
0.000754
AFFX-BioB-M_at
485.7
P
0.000195
AFFX-BioB-3_at
241
P
0.000147
AFFX-BioC-5_at
710.6
P
0.000147
AFFX-BioC-3_at
577.7
P
0.000052
AFFX-BioDn-5_at
723.4
P
0.000044
AFFX-BioDn-3_at
3529.5
P
0.000081
AFFX-CreX-5_at
7937.6
P
0.000052
AFFX-CreX-3_at
10061.3
P
0.000044
AFFX-DapX-5_at
14451.2
P
0.000044
AFFX-DapX-M_at
15039
P
0.000044
AFFX-DapX-3_at
15965
P
0.000044
AFFX-LysX-5_at
6.2
A
0.340661
AFFX-LysX-M_at
6
A
0.712257
AFFX-LysX-3_at
18.7
A
0.131361
AFFX-PheX-5_at
2
A
0.916408
AFFX-PheX-M_at
2
A
0.973889
AFFX-PheX-3_at
18.1
A
0.52976
AFFX-ThrX-5_at
3.4
A
0.921998
AFFX-ThrX-M_at
4
A
0.737173
45101 1205 Kbytes .Supplementary data files not provided
