GEO Accession viewer

NCBI > GEO > Accession Display

Not logged in | Login

GEO help: Mouse over screen elements for information.

Sample GSM67373

Query DataSets for GSM67373

Status

Public on Sep 26, 2005

Title

del337TR replicate 5

Sample type

RNA

Source name

Mouse heart

Organism

Mus musculus

Characteristics

deletion in 337TR

Extracted molecule

total RNA

Extraction protocol

Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.

Label

biotin

Label protocol

Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.

Hybridization protocol

standard Affymetrix procedures

Scan protocol

standard Affymetrix procedures

Description

deletion in 337TR

Data processing

Robust Multichip Analysis (RMA)

Submission date

Aug 05, 2005

Last update date

Sep 26, 2005

Contact name

Norman Buroker

E-mail(s)

Norman.Buroker@seattlechildrens.org

Organization name

Seattle Children's Hospital

Lab

Portman

Street address

4800 Sand Point Way N.E.

City

Seattle

State/province

ZIP/Postal code

98105

Country

USA

Platform ID

GPL1261

Series (1)

GSE3067

Resistance to thyroid hormone (RTH) and PPARa activation

Data table header descriptions
ID_REF
VALUE	MAS5-calculated Signal intensity
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
AFFX-BioB-5_at	269.2	P	0.000754
AFFX-BioB-M_at	464.6	P	0.000147
AFFX-BioB-3_at	230.9	P	0.000754
AFFX-BioC-5_at	739.2	P	0.000081
AFFX-BioC-3_at	565.6	P	0.000044
AFFX-BioDn-5_at	751	P	0.000044
AFFX-BioDn-3_at	4110	P	0.000127
AFFX-CreX-5_at	10543.1	P	0.000052
AFFX-CreX-3_at	11188.1	P	0.000044
AFFX-DapX-5_at	30.4	A	0.108959
AFFX-DapX-M_at	20	A	0.39692
AFFX-DapX-3_at	43.4	A	0.205732
AFFX-LysX-5_at	3.4	A	0.686292
AFFX-LysX-M_at	10.5	A	0.686277
AFFX-LysX-3_at	37.5	A	0.095667
AFFX-PheX-5_at	1793.8	P	0.000044
AFFX-PheX-M_at	2159.1	P	0.000052
AFFX-PheX-3_at	3606.1	P	0.000044
AFFX-ThrX-5_at	10.2	A	0.749223
AFFX-ThrX-M_at	3.7	A	0.760937

Total number of rows: 45101

Table truncated, full table size 1204 Kbytes.

Supplementary data files not provided