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Sample GSM67386 Query DataSets for GSM67386
Status Public on Sep 26, 2005
Title del337 with WY14643 replicate 4
Sample type RNA
 
Source name Mouse heart
Organism Mus musculus
Characteristics del337 treated with WY14643
Extracted molecule total RNA
Extraction protocol Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description del337 treated with WY14643
Data processing Robust Multichip Analysis (RMA)
 
Submission date Aug 05, 2005
Last update date Sep 26, 2005
Contact name Norman Buroker
E-mail(s) Norman.Buroker@seattlechildrens.org
Organization name Seattle Children's Hospital
Lab Portman
Street address 4800 Sand Point Way N.E.
City Seattle
State/province WA
ZIP/Postal code 98105
Country USA
 
Platform ID GPL1261
Series (1)
GSE3067 Resistance to thyroid hormone (RTH) and PPARa activation

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 555.5 P 0.000754
AFFX-BioB-M_at 1031 P 0.000052
AFFX-BioB-3_at 511.6 P 0.000044
AFFX-BioC-5_at 1293.9 P 0.00007
AFFX-BioC-3_at 1180.2 P 0.000044
AFFX-BioDn-5_at 1488.4 P 0.000044
AFFX-BioDn-3_at 6487 P 0.00006
AFFX-CreX-5_at 17855.3 P 0.000052
AFFX-CreX-3_at 17298.9 P 0.000044
AFFX-DapX-5_at 45.4 P 0.015183
AFFX-DapX-M_at 83.5 A 0.067678
AFFX-DapX-3_at 100.2 P 0.004998
AFFX-LysX-5_at 20.3 A 0.455413
AFFX-LysX-M_at 26 A 0.544587
AFFX-LysX-3_at 47.9 P 0.012547
AFFX-PheX-5_at 2.6 A 0.971543
AFFX-PheX-M_at 2.2 A 0.969024
AFFX-PheX-3_at 13.1 A 0.48511
AFFX-ThrX-5_at 15.2 A 0.737173
AFFX-ThrX-M_at 21.1 A 0.529762

Total number of rows: 45101

Table truncated, full table size 1203 Kbytes.




Supplementary data files not provided

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