|
Status |
Public on Apr 24, 2024 |
Title |
Schwann Cells, NF2-WT |
Sample type |
SRA |
|
|
Source name |
Schwannoma
|
Organism |
Homo sapiens |
Characteristics |
tissue: Schwannoma cell type: Schwann Cells genotype: NF2-WT
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using an RNeasy kit from Qiagen RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.
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|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Data processing |
Data were processed on the Galaxy server Adapters were trimmed with "Trim Galore !" Reads were aligned to UCSC hg38 Reads per sgRNA were counted using custom software ("count_features") available at https://github.com/aurelioteleman/Teleman-Lab Assembly: hg38 Supplementary files format and content: tab-delimited file containing raw read counts for all samples as well as read counts normalized to library size
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|
|
Submission date |
Dec 01, 2022 |
Last update date |
Apr 24, 2024 |
Contact name |
Aurelio A Teleman |
Organization name |
German Cancer Research Center (DKFZ)
|
Street address |
Im Neuenheimer Feld 580
|
City |
Heidelberg |
ZIP/Postal code |
69120 |
Country |
Germany |
|
|
Platform ID |
GPL11154 |
Series (2) |
GSE219135 |
Screen for genes showing synthetic lethality with NF2 in Schwannoma [RNA-Seq] |
GSE219141 |
Screen for genes showing synthetic lethality with NF2 in Schwannoma |
|
Relations |
BioSample |
SAMN31956648 |
SRA |
SRX18443478 |