|
| Status |
Public on Apr 24, 2024 |
| Title |
Schwann Cells, NF2-KO |
| Sample type |
SRA |
| |
|
| Source name |
Schwannoma
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Schwannoma
cell type: Schwann Cells
genotype: NF2-KO
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using an RNeasy kit from Qiagen
RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Data were processed on the Galaxy server
Adapters were trimmed with "Trim Galore !"
Reads were aligned to UCSC hg38
Reads per sgRNA were counted using custom software ("count_features") available at https://github.com/aurelioteleman/Teleman-Lab
Assembly: hg38
Supplementary files format and content: tab-delimited file containing raw read counts for all samples as well as read counts normalized to library size
|
| |
|
| Submission date |
Dec 01, 2022 |
| Last update date |
Apr 24, 2024 |
| Contact name |
Aurelio A Teleman |
| Organization name |
German Cancer Research Center (DKFZ)
|
| Street address |
Im Neuenheimer Feld 580
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL11154 |
| Series (2) |
| GSE219135 |
Screen for genes showing synthetic lethality with NF2 in Schwannoma [RNA-Seq] |
| GSE219141 |
Screen for genes showing synthetic lethality with NF2 in Schwannoma |
|
| Relations |
| BioSample |
SAMN31956647 |
| SRA |
SRX18443479 |
