|
Status |
Public on May 27, 2011 |
Title |
Control ESC line treated with DMSO - replicate 1 |
Sample type |
RNA |
|
|
Source name |
ES line transfected with control short hairpin, treated with DMSO
|
Organism |
Homo sapiens |
Characteristics |
cell line: ES[4] replicate: 1 modification: transfected with control short hairpin, treated with DMSO
|
Growth protocol |
The human embryonic stem cell line ES[4] was grown in matrigel coated plates and in the presence of irradiated MEFs conditioned media supplemented with FGF.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using miRNeasy Mini Kit (Qiagen).
|
Label |
Cy3
|
Label protocol |
100 ng of total RNA was labeled using LowInputQuick Amp Labeling kit (Agilent 5190-2305) following manufacturer instructions.
|
|
|
Hybridization protocol |
Labeled cRNA was hybridized to the Agilent SurePrint G3 Human gene expression 8x60K microarray according to the manufacturer's protocol.
|
Scan protocol |
The arrays were scanned on an Agilent G2565CA microarray scanner at 100% PMT and 3 um resolution.
|
Data processing |
Intensity data was extracted using the Feature Extraction software (Agilent).
|
|
|
Submission date |
Mar 11, 2011 |
Last update date |
Nov 15, 2012 |
Contact name |
Stephanie Boue |
Organization name |
CMRB
|
Street address |
Dr Aiguader 88
|
City |
Barcelona |
ZIP/Postal code |
08003 |
Country |
Spain |
|
|
Platform ID |
GPL14550 |
Series (1) |
GSE24844 |
LSD1/KDM1 regulates the balance between self-renewal and differentiation in human embryonic stem cells. |
|