|
| Status |
Public on May 27, 2011 |
| Title |
LSD1 knock-down ESC line overexpressing wild type LSD1 - replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
LSD1 knock-down ESC line overexpressing wild type LSD1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: ES[4]
replicate: 2
|
| Growth protocol |
The human embryonic stem cell line ES[4] was grown in matrigel coated plates and in the presence of irradiated MEFs conditioned media supplemented with FGF.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using miRNeasy Mini Kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
100 ng of total RNA was labeled using LowInputQuick Amp Labeling kit (Agilent 5190-2305) following manufacturer instructions.
|
| |
|
| Hybridization protocol |
Labeled cRNA was hybridized to the Agilent SurePrint G3 Human gene expression 8x60K microarray according to the manufacturer's protocol.
|
| Scan protocol |
The arrays were scanned on an Agilent G2565CA microarray scanner at 100% PMT and 3 um resolution.
|
| Data processing |
Intensity data was extracted using the Feature Extraction software (Agilent).
|
| |
|
| Submission date |
Mar 11, 2011 |
| Last update date |
Nov 15, 2012 |
| Contact name |
Stephanie Boue |
| Organization name |
CMRB
|
| Street address |
Dr Aiguader 88
|
| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
|
| Platform ID |
GPL14550 |
| Series (1) |
| GSE24844 |
LSD1/KDM1 regulates the balance between self-renewal and differentiation in human embryonic stem cells. |
|
