|
| Status |
Public on Sep 30, 2011 |
| Title |
siPBX1 treated with E2 rep2 |
| Sample type |
RNA |
| |
|
| Source name |
MCF7 breast cancer cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF7 breast cancer cells
era status: positive
genotype/variation: PBX1 knock down
agent: estradiol (E2)
|
| Treatment protocol |
72h post transfection with the appropriate siRNA, cells were stimulated with 10nM E2 or DMSO for 3h prior RNA extraction
|
| Growth protocol |
Cells were grown in stripped media (DMEM w/o phenol, CDT treated serum 10%, glutamine and penicillin/streptomycin).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with with QIAGEN RNeasy kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser. Efficient knock down was validated with RT-qPCR.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
PBX1_E2
replicate 2
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Mar 17, 2011 |
| Last update date |
Sep 30, 2011 |
| Contact name |
luca magnani |
| E-mail(s) |
luca.magnani@dartmouth.edu
|
| Phone |
6036539932
|
| Fax |
6036539952
|
| Organization name |
Dartmouth College
|
| Department |
Genetics
|
| Lab |
Lupien
|
| Street address |
NCCC one medical drive
|
| City |
Lebanon |
| State/province |
NH |
| ZIP/Postal code |
03756 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE28006 |
The pioneer factor PBX1 guides a distinct ERa signaling in breast cancer [mRNA profiling] |
| GSE28008 |
The pioneer factor PBX1 guides a distinct ERa signaling in breast cancer |
|
