|
Status |
Public on Jul 27, 2023 |
Title |
LNCaP-CPA-4hr-R1 |
Sample type |
RNA |
|
|
Source name |
LNCaP cell tissue culture
|
Organism |
Homo sapiens |
Characteristics |
cell type: Prostate cancer LNCaP cell line treatment: CPA-4hr
|
Treatment protocol |
Cells were either untreated or treated for 2 hours, 4 hours, 8 hours, or 24 hours with Vehicle (Ethanol), CPA (10µM of cyproterone acetate), or DHT (1nM of Dihydrotestosterone). Two repeats for each setting.
|
Growth protocol |
LNCaP cells were cultured in androgen-depleted medium (RPMI1640 + 5% of Charcoal/Dextran treated Fetal Bovine Serum) for 2 days.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated with TriZOL reagent.
|
Label |
Biotin-labeled cRNA
|
Label protocol |
Biotin-labeled cRNA was generated. hybridized to Affymetrix U133A oligonucleotide microarrays as described
|
|
|
Hybridization protocol |
Hybridized to Affymetrix U133A oligonucleotide microarrays as described (PMID: 10521349).
|
Scan protocol |
N/A
|
Description |
LNCaP cells in androgen depleted-medium, treated with CPA (10µM of cyproterone acetate) for 4hrs, repeat 1
|
Data processing |
The expression files were normalized across the arrays, and genes expressed below 10 were filtered out.
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|
|
Submission date |
Jan 19, 2023 |
Last update date |
Jul 27, 2023 |
Contact name |
Shaoyong Chen |
E-mail(s) |
shaoyong_chen@hotmail.com
|
Phone |
16178660935
|
Organization name |
Boston BIB
|
Department |
Hem/Onc
|
Lab |
Balk
|
Street address |
3 Blackfan Circle, CLS-432
|
City |
Boston |
State/province |
Massachusetts |
ZIP/Postal code |
02215 |
Country |
USA |
|
|
Platform ID |
GPL96 |
Series (1) |
GSE223251 |
Gene expression profiling in prostate cancer cell line LNCaP. |
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