|
| Status |
Public on Jul 27, 2023 |
| Title |
LNCaP-DHT-8hr-R2 |
| Sample type |
RNA |
| |
|
| Source name |
LNCaP cell tissue culture
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Prostate cancer LNCaP cell line
treatment: DHT-8hr
|
| Treatment protocol |
Cells were either untreated or treated for 2 hours, 4 hours, 8 hours, or 24 hours with Vehicle (Ethanol), CPA (10µM of cyproterone acetate), or DHT (1nM of Dihydrotestosterone). Two repeats for each setting.
|
| Growth protocol |
LNCaP cells were cultured in androgen-depleted medium (RPMI1640 + 5% of Charcoal/Dextran treated Fetal Bovine Serum) for 2 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with TriZOL reagent.
|
| Label |
Biotin-labeled cRNA
|
| Label protocol |
Biotin-labeled cRNA was generated. hybridized to Affymetrix U133A oligonucleotide microarrays as described
|
| |
|
| Hybridization protocol |
Hybridized to Affymetrix U133A oligonucleotide microarrays as described (PMID: 10521349).
|
| Scan protocol |
N/A
|
| Description |
LNCaP cells in androgen depleted-medium, treated with DHT (1nM of Dihydrotestosterone) for 8hrs, repeat 2
|
| Data processing |
The expression files were normalized across the arrays, and genes expressed below 10 were filtered out.
|
| |
|
| Submission date |
Jan 19, 2023 |
| Last update date |
Jul 27, 2023 |
| Contact name |
Shaoyong Chen |
| E-mail(s) |
shaoyong_chen@hotmail.com
|
| Phone |
16178660935
|
| Organization name |
Boston BIB
|
| Department |
Hem/Onc
|
| Lab |
Balk
|
| Street address |
3 Blackfan Circle, CLS-432
|
| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE223251 |
Gene expression profiling in prostate cancer cell line LNCaP. |
|
