|
| Status |
Public on Apr 14, 2011 |
| Title |
Chromatin accessibility assay of CD19 Primary Cells; DS17186 |
| Sample type |
SRA |
| |
|
| Source name |
flow sorted CD19+ Cells, year 21; DS17186
|
| Organism |
Homo sapiens |
| Characteristics |
sample alias: CD19.RO_01721
sample common name: CD19 Primary Cells
molecule: genomic DNA
disease: None
biomaterial_provider: FHCRC HEIMFELD
biomaterial_type: Primary Cell
cell_type: CD19 Primary Cells
markers: CD19+
donor_id: RO 01679
donor_age: year 21
donor_health_status: NA
donor_sex: Male
donor_ethnicity: Caucasian
passage_if_expanded: NA
experiment_type: Chromatin Accessibility
extraction_protocol: Qiagen minElut
dnase_protocol: Stamlab DNase Protocol, Sabo, P. J. et al. Nat Methods 3, 511-518 (2006)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Library construction protocol: Single read - Illumina
|
| |
|
| Library strategy |
DNase-Hypersensitivity |
| Library source |
genomic |
| Library selection |
DNAse |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Description |
sample_term_id: CL_0000236
assay_term_id: OBI_0001853
nucleic_acid_term_id: SO_0000352
Design description: Chromatin accessibility determination via DNase-seq
Library name: DS17186
EDACC Genboree Experiment Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FUniversity%20of%20Washington%2FEXPERIMENT%2FEDACC.6966
EDACC Genboree Sample Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FUniversity%20of%20Washington%2FSAMPLE%2FEDACC.6965
****************
For data usage terms and conditions, please refer to:
http://www.drugabuse.gov/funding/funding-opportunities/nih-common-fund/epigenomics-data-access-policies
****************
|
| Data processing |
**********************************************************************
ANALYSIS FILE NAME: GSM701492_UW.CD19_Primary_Cells.ChromatinAccessibility.RO_01679.DS17186.bed
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: DS17186.hg19.level.1.release.4
ANALYSIS TITLE: Mapping of CD19 Primary Cells Chromatin Accessibility Data
ANALYSIS DESCRIPTION: Illumina reads produced by DNAse-Seq on CD19 Primary Cells, Donor RO 01679, Library DS17186 were mapped to the human genome using Pash.
ANALYSIS TYPE: REFERENCE_ALIGNMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.7690
DATA_ANALYSIS_LEVEL: 1
EXPERIMENT_TYPE: Chromatin Accessibility
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: Pash
SOFTWARE_VERSION: 3.0
MAXIMUM_ALIGNMENT_LENGTH: Read length
MISMATCHES_ALLOWED: 10% of read length
ALIGNMENTS_ALLOWED: 1
TREATMENT_OF_MULTIPLE_ALIGNMENTS: If a read maps to more than 1 position it is removed from consideration.
TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: None
ALIGNMENT_POSTPROCESSING: None
RELEASE_NUMBER: Human Epigenome Atlas 4
QUALITY SCORES:
NUMBER_OF_MAPPED_READS: 27,493,357
NUMBER_OF_CHROMATIN_ACCESSIBILITY_EXPERIMENTS_SCORED_IN_THIS_RELEASE: 54
FINDPEAKS_SCORE: 0.4227
FINDPEAKS_PERCENTILE: 69
HOTSPOT_SCORE: 0.427
HOTSPOT_PERCENTILE: 67
IROC_SCORE: 0.9951
IROC_PERCENTILE: 57
POISSON_SCORE: 0.457
POISSON_PERCENTILE: 59
MAXIMUM_REPLICATE_CORRELATION: 1.0
**********************************************************************
ANALYSIS FILE NAME: GSM701492_UW.CD19_Primary_Cells.ChromatinAccessibility.RO_01679.DS17186.wig
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: DS17186.hg19.level.2.release.4
ANALYSIS TITLE: Raw Signal Density Graphs of CD19 Primary Cells Chromatin Accessibility Data
ANALYSIS DESCRIPTION: Illumina DNAse-Seq read mappings from CD19 Primary Cells, Donor RO 01679, Library DS17186 were processed into density graphs of raw signal representing the aligned read density.
ANALYSIS TYPE: ABUNDANCE_MEASUREMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.7744
DATA_ANALYSIS_LEVEL: 2
EXPERIMENT_TYPE: Chromatin Accessibility
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: In house programs and scripts
SOFTWARE_VERSION: NA
READ_EXTENSION: 0bp
GENOMIC_WINDOW: 150bp
TREATMENT_OF_REGIONS_PRONE_TO_MULTIPLE_ALIGNMENTS: None
RELEASE_NUMBER: Human Epigenome Atlas 4
BROWSER_TRACK_NAME: CD19 DNase 21 86
BROWSER_TRACK_DESCRIPTION: UW CD19 Primary Cells DNase Hypersensitivity Donor RO 01679 Library DS17186 EA Release 4
QUALITY SCORES:
NUMBER_OF_MAPPED_READS: 27,493,357
NUMBER_OF_CHROMATIN_ACCESSIBILITY_EXPERIMENTS_SCORED_IN_THIS_RELEASE: 52
FINDPEAKS_SCORE: 0.4227
FINDPEAKS_PERCENTILE: 69
HOTSPOT_SCORE: 0.427
HOTSPOT_PERCENTILE: 67
IROC_SCORE: 0.9951
IROC_PERCENTILE: 57
POISSON_SCORE: 0.457
POISSON_PERCENTILE: 59
MAXIMUM_REPLICATE_CORRELATION: 1.0
**********************************************************************
|
| |
|
| Submission date |
Apr 04, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Northwest REMC |
| E-mail(s) |
rharris1@bcm.tmc.edu
|
| Organization name |
University of Washington
|
| Street address |
-
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195 |
| Country |
USA |
| |
|
| Platform ID |
GPL10999 |
| Series (1) |
| GSE18927 |
University of Washington Human Reference Epigenome Mapping Project |
|
| Relations |
| SRA |
SRX055156 |
| BioSample |
SAMN00254246 |
| Named Annotation |
GSM701492_UW.CD19_Primary_Cells.ChromatinAccessibility.RO_01679.DS17186.wig.gz |
