|
| Status |
Public on May 16, 2011 |
| Title |
WT, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
wild-type brain
|
| Organism |
Mus musculus |
| Characteristics |
age: 1 mo
tissue: brain
genotype: wild-type
gender: Male
genetic background: mixed 129S2/Sv and C57BL/6J
|
| Growth protocol |
Brains dissected from Rbfox1-KO mice or WT mice, 1 month of age
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mice were genotyped and whole brains were dissected from 3 mutant and 3 wild-type animals. Total RNA was extracted using Trizol and samples were treated with RiboMinus™ Transcriptome Isolation Kit (Invitrogen). cDNA synthesis and amplification was performed using the GeneChip® Whole Transcript Sense Target Labeling and Control Reagents kit (Affymetrix).
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Target Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Hybridization was performed as specified, with the GeneChip Hybridization Wash and Stain Kit (Affymetrix)
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Data processing |
Using Affymetrix package (Affy Power Tools) apt-probeset-summarize. Iter-plier algorithm used to quantify probesets.
GPL13185_mjay.pgf
GPL13185_mjay.clf
|
| |
|
| Submission date |
Apr 06, 2011 |
| Last update date |
May 16, 2011 |
| Contact name |
Lily Shiue |
| E-mail(s) |
lshiue@ucsc.edu
|
| Phone |
831-459-2612
|
| Organization name |
UCSC
|
| Department |
MCD Biology
|
| Lab |
Ares
|
| Street address |
1156 High Street
|
| City |
Santa Cruz |
| State/province |
CA |
| ZIP/Postal code |
95064 |
| Country |
USA |
| |
|
| Platform ID |
GPL13185 |
| Series (1) |
| GSE28421 |
The splicing regulator Rbfox1 (A2BP1) controls neuronal excitation in the mammalian brain |
|
