|
Status |
Public on Apr 12, 2023 |
Title |
E. coli P4-NR2 |
Sample type |
RNA |
|
|
Source name |
Mammary gland, E. coli challenged
|
Organism |
Mus musculus |
Characteristics |
tissue: mammary gland treatment: E. coli challenged
|
Treatment protocol |
Mice were infected with E. coli, Strep. uberis or M. bovis bacteria by intramammary infusion. After 24 or 48 hours, mammary tissues were sampled for RNA extraction.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the GeneElute Mammalian Total RNA Miniprep Kit (Sigma-Aldrich, Rehovot, Israel) combined with on-Column DNase I Digestion Set (Sigma) and stored at -80C.
|
Label |
None
|
Label protocol |
Labeling was performed according to manufacturer's instructions.
|
|
|
Hybridization protocol |
Hybridization was performed according to manufacturer's instructions.
|
Scan protocol |
nCounter Sprint profiler was used for scanning and image acquisition.
|
Data processing |
Data was analyzed using nSolver™ analysis software version 4.0
|
|
|
Submission date |
Feb 12, 2023 |
Last update date |
Apr 12, 2023 |
Contact name |
Nahum Yehuda Shpigel |
E-mail(s) |
nahum.shpigel@mail.huji.ac.il
|
Phone |
0548820526
|
Organization name |
Hebrew University
|
Department |
Veterinary
|
Lab |
Shpigelab
|
Street address |
POB 12
|
City |
Rehovot |
State/province |
Please Select… |
ZIP/Postal code |
76100 |
Country |
Israel |
|
|
Platform ID |
GPL25266 |
Series (1) |
GSE225127 |
Immune profiling of experimental murine mastitis |
|