|
| Status |
Public on Apr 12, 2023 |
| Title |
E. coli P4-NR3 |
| Sample type |
RNA |
| |
|
| Source name |
Mammary gland, E. coli challenged
|
| Organism |
Mus musculus |
| Characteristics |
tissue: mammary gland
treatment: E. coli challenged
|
| Treatment protocol |
Mice were infected with E. coli, Strep. uberis or M. bovis bacteria by intramammary infusion. After 24 or 48 hours, mammary tissues were sampled for RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the GeneElute Mammalian Total RNA Miniprep Kit (Sigma-Aldrich, Rehovot, Israel) combined with on-Column DNase I Digestion Set (Sigma) and stored at -80C.
|
| Label |
None
|
| Label protocol |
Labeling was performed according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Hybridization was performed according to manufacturer's instructions.
|
| Scan protocol |
nCounter Sprint profiler was used for scanning and image acquisition.
|
| Data processing |
Data was analyzed using nSolver™ analysis software version 4.0
|
| |
|
| Submission date |
Feb 12, 2023 |
| Last update date |
Apr 12, 2023 |
| Contact name |
Nahum Yehuda Shpigel |
| E-mail(s) |
nahum.shpigel@mail.huji.ac.il
|
| Phone |
0548820526
|
| Organization name |
Hebrew University
|
| Department |
Veterinary
|
| Lab |
Shpigelab
|
| Street address |
POB 12
|
| City |
Rehovot |
| State/province |
Please Select… |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platform ID |
GPL25266 |
| Series (1) |
| GSE225127 |
Immune profiling of experimental murine mastitis |
|
