|
| Status |
Public on Feb 14, 2023 |
| Title |
iPSC_DRENEA_5 |
| Sample type |
SRA |
| |
|
| Source name |
iPSCs
|
| Organism |
Homo sapiens |
| Characteristics |
cell lines: FSA0006,TOB0421,MBE1006
hashtag antibodies: NA
time: Village
cryopreserved: Fresh
cell type: iPSCs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Approximately 32,000 cells were loaded onto the Chromium Single Cell Chip B (10X Genomics; Catalog Number: PN-1000073) in order to capture 20,000 single cells with the Gel Bead Kit V3.0 (10x Genomics; Catalog Number: PN-1000076). GEM generation, barcoding, cDNA amplification, and library construction were performed according to the 10x Genomics Chromium User Guide (CG000183).
Libraries were prepared with the Single Cell 3' V3.0 Library and Gel Bead Kit (10x Genomics; Catalog Number: PN-1000077 and PN-1000078).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
10X Genomics Chromium Controller
Single cell sequencing sequencing of genotype and hashtag pooled samples
|
| Data processing |
cellranger was used to process the reads for quality, aligning to the genome and counting the UMIs per gene per cell
Assembly: hg38
Supplementary files format and content: cellranger filtered matrxi output
|
| |
|
| Submission date |
Feb 14, 2023 |
| Last update date |
Feb 14, 2023 |
| Contact name |
Drew Neavin |
| E-mail(s) |
d.neavin@garvan.org.au
|
| Organization name |
Garvan Institute of Medical Research
|
| Department |
Cellular Science
|
| Street address |
384 Victoria St
|
| City |
Darlinghurst |
| State/province |
New South Wales |
| ZIP/Postal code |
2010 |
| Country |
Australia |
| |
|
| Platform ID |
GPL11154 |
| Series (2) |
| GSE225278 |
Village In a Dish: A Model System for Population-scale hiPSC Studies [scRNA-Seq] |
| GSE225282 |
Village In a Dish: A Model System for Population-scale hiPSC Studies |
|
| Relations |
| BioSample |
SAMN33285981 |
| SRA |
SRX19364974 |
