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Sample GSM7045928 Query DataSets for GSM7045928
Status Public on Feb 18, 2024
Title 51.4.2 LCN2
Sample type protein
 
Source name BMDMs
Organism Mus musculus
Characteristics cell type: bone marrow derived macrophages
genotype: C57BL/6 wildtype
gender: female
treament: LCN2
Treatment protocol From start of differentiation, cells were kept with 150 ng/ml LCN2 or vehicle.
Growth protocol Bone marrow was isolated from five wild-type C57BL/6 mice, erythrocytes were lyzed and residual cells were plated in RPMI+10%FCS+1%PenStrep+20ng/ml rmCSF at 3.5x10^6 cells per 10cm dish. Cells were cultivated until d7, when they were treated with LPS and IFNg. 16 hours after stimulation, cell pellets were harvested.
Extracted molecule protein
Extraction protocol Cell pellets were frozen, shipped, and lyzed in 25 µl buffer with protease and phosphatase inhibitors.
Label fluorescently labelled anti phospho antibody
Label protocol maximum 15 µl was used as per proprietary protocol of PamGene
 
Hybridization protocol sample is pumped through porous membrane
Scan protocol when the solution is underneath the array, images are taken at several exposure times
Description 180-205 36
Protein lysates with active kinases, phosphorylation of spotted peptides on chip detected with fluorescently labeled anti-phospho antibody
Data processing images are used by the BioNavigator software to calculate signal values for each phosphosite.
 
Submission date Feb 15, 2023
Last update date Feb 18, 2024
Contact name Robert Zeiser
Organization name University Medical Center Freiburg
Department Interdisziplinäres Tumorzentrum
Street address Hugstetter Straße 53
City Freiburg
ZIP/Postal code 79106
Country Germany
 
Platform ID GPL33127
Series (1)
GSE225341 Effect of recombinant murine Lipocalin-2 (LCN2) on murine macrophages

Data table header descriptions
ID_REF
VALUE log transformed signal minus background

Data table
ID_REF VALUE
41_654_666 11.66820
ACHD_383_395 8.18602
AKT1_170_182 6.44422
AKT1_309_321_C310S 9.74043
AKT1_320_332 9.51358
ANXA1_14_26 0
ANXA2_17_29 9.56839
ARAF_297_307 10.81821
ART_004_EAIYAAPFAKKKXC 13.16553
B3AT_39_51 8.38702
BTLA_252_262 10.53826
C1R_199_211 7.94522
CALM_93_105 7.28226
CALM_95_107 7.98525
CBL_693_705 7.98657
CD28_185_197 7.91427
CD28_203_215 9.34498
CD3E_182_194 13.42469
CD3E_193_205 9.15615
CD3Z_105_117 13.81097

Total number of rows: 167

Table truncated, full table size 3 Kbytes.




Supplementary data files not provided
Processed data are available on Series record

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