|
| Status |
Public on Apr 12, 2023 |
| Title |
Type 2 diabetes patient 178 |
| Sample type |
genomic |
| |
|
| Source name |
Type 2 diabetes
|
| Organism |
Homo sapiens |
| Characteristics |
age: 44
gender: Male
bmi: 29.54
complication type: Retinopathy, Neuropathy, Nephropathy
disease state: Type 2 diabetes
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Extraction of genomic DNA from fresh frozen tissue was performed using the Qiagen DNA extraction kit per the manufacturer’s instructions.
|
| Label |
Cy3, Cy5
|
| Label protocol |
300 ng of genomic DNA was amplified in an overnigh reaction at 37 ˚C using multi-sample amplification mix 1 (MA1), multi-sample amplification mix 2 (MA2), and multi-sample amplification master mix (MSM). After incubation, the amplified DNA was fragmented with fragmaentation solution (FMS), percipitated with 2-propanol and percipitation solution (PM1), and resuspended in resuspension, hybridization, and wash solution (RA1).
|
| |
|
| Hybridization protocol |
RA1 resuspended DNA was loaded onto BeadChips arrays.After overnight incubation at 48˚C, single-base extension and allele-specific staining was performed on a Teflow chamber rack system (Tecan, Maennedorf, Switzerland).
|
| Scan protocol |
After allele-specific staining BeadChip arrays were coated with XStain BeadChip solution 4 (XC4) and wash buffer (PB1), dried for 1 hour and scanned on iScan System microarray scanner (Illumina).
|
| Description |
Genomic DNA extracted from the blood of type 2 diabetes patients was genotyped using Infinium Exome BeadChip (Illumina).
DXB_DDC_21_7152388_00931
|
| Data processing |
Genomic DNA extracted from the blood of type 2 diabetes patients was genotyped using Infinium Exome BeadChip (Illumina).
|
| |
|
| Submission date |
Feb 24, 2023 |
| Last update date |
Apr 12, 2023 |
| Contact name |
Habiba Al Safar |
| E-mail(s) |
habiba.alsafar@ku.ac.ae
|
| Phone |
+971 2 312 3030
|
| Organization name |
Khalifa University
|
| Department |
Biomedical Engineering
|
| Lab |
Biotechnology
|
| Street address |
Shakhbout Bin Sultan St
|
| City |
Abu Dhabi |
| State/province |
Abu Dhabi |
| ZIP/Postal code |
Zone 1 |
| Country |
United Arab Emirates |
| |
|
| Platform ID |
GPL26511 |
| Series (1) |
| GSE226084 |
Microvascular and Macrovascuar Complications of Type 2 Diabetes Mellitus: Exome Wide Association Analyses |
|
