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Sample GSM7063168 Query DataSets for GSM7063168
Status Public on Apr 12, 2023
Title Type 2 diabetes patient 214
Sample type genomic
 
Source name Type 2 diabetes
Organism Homo sapiens
Characteristics age: 62
gender: Female
bmi: 25.78
complication type: No complications
disease state: Type 2 diabetes
Extracted molecule genomic DNA
Extraction protocol Extraction of genomic DNA from fresh frozen tissue was performed using the Qiagen DNA extraction kit per the manufacturer’s instructions.
Label Cy3, Cy5
Label protocol 300 ng of genomic DNA was amplified in an overnigh reaction at 37 ˚C using multi-sample amplification mix 1 (MA1), multi-sample amplification mix 2 (MA2), and multi-sample amplification master mix (MSM). After incubation, the amplified DNA was fragmented with fragmaentation solution (FMS), percipitated with 2-propanol and percipitation solution (PM1), and resuspended in resuspension, hybridization, and wash solution (RA1).
 
Hybridization protocol RA1 resuspended DNA was loaded onto BeadChips arrays.After overnight incubation at 48˚C, single-base extension and allele-specific staining was performed on a Teflow chamber rack system (Tecan, Maennedorf, Switzerland).
Scan protocol After allele-specific staining BeadChip arrays were coated with XStain BeadChip solution 4 (XC4) and wash buffer (PB1), dried for 1 hour and scanned on iScan System microarray scanner (Illumina).
Description Genomic DNA extracted from the blood of type 2 diabetes patients was genotyped using Infinium Exome BeadChip (Illumina).
DXB_TWR_21_7076461_00668
Data processing Genomic DNA extracted from the blood of type 2 diabetes patients was genotyped using Infinium Exome BeadChip (Illumina).
 
Submission date Feb 24, 2023
Last update date Apr 12, 2023
Contact name Habiba Al Safar
E-mail(s) habiba.alsafar@ku.ac.ae
Phone +971 2 312 3030
Organization name Khalifa University
Department Biomedical Engineering
Lab Biotechnology
Street address Shakhbout Bin Sultan St
City Abu Dhabi
State/province Abu Dhabi
ZIP/Postal code Zone 1
Country United Arab Emirates
 
Platform ID GPL26511
Series (1)
GSE226084 Microvascular and Macrovascuar Complications of Type 2 Diabetes Mellitus: Exome Wide Association Analyses

Supplementary file Size Download File type/resource
GSM7063168_204070120057_R07C02_Grn.idat.gz 2.0 Mb (ftp)(http) IDAT
GSM7063168_204070120057_R07C02_Red.idat.gz 2.0 Mb (ftp)(http) IDAT
Processed data are available on Series record

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