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Status |
Public on Apr 12, 2023 |
Title |
Type 2 diabetes patient 281 |
Sample type |
genomic |
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|
Source name |
Type 2 diabetes
|
Organism |
Homo sapiens |
Characteristics |
age: 61 gender: Female bmi: 28.91 complication type: No complications disease state: Type 2 diabetes
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Extraction of genomic DNA from fresh frozen tissue was performed using the Qiagen DNA extraction kit per the manufacturer’s instructions.
|
Label |
Cy3, Cy5
|
Label protocol |
300 ng of genomic DNA was amplified in an overnigh reaction at 37 ˚C using multi-sample amplification mix 1 (MA1), multi-sample amplification mix 2 (MA2), and multi-sample amplification master mix (MSM). After incubation, the amplified DNA was fragmented with fragmaentation solution (FMS), percipitated with 2-propanol and percipitation solution (PM1), and resuspended in resuspension, hybridization, and wash solution (RA1).
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Hybridization protocol |
RA1 resuspended DNA was loaded onto BeadChips arrays.After overnight incubation at 48˚C, single-base extension and allele-specific staining was performed on a Teflow chamber rack system (Tecan, Maennedorf, Switzerland).
|
Scan protocol |
After allele-specific staining BeadChip arrays were coated with XStain BeadChip solution 4 (XC4) and wash buffer (PB1), dried for 1 hour and scanned on iScan System microarray scanner (Illumina).
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Description |
Genomic DNA extracted from the blood of type 2 diabetes patients was genotyped using Infinium Exome BeadChip (Illumina). DXB_DDC_21_13420348_00581
|
Data processing |
Genomic DNA extracted from the blood of type 2 diabetes patients was genotyped using Infinium Exome BeadChip (Illumina).
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Submission date |
Feb 24, 2023 |
Last update date |
Apr 12, 2023 |
Contact name |
Habiba Al Safar |
E-mail(s) |
habiba.alsafar@ku.ac.ae
|
Phone |
+971 2 312 3030
|
Organization name |
Khalifa University
|
Department |
Biomedical Engineering
|
Lab |
Biotechnology
|
Street address |
Shakhbout Bin Sultan St
|
City |
Abu Dhabi |
State/province |
Abu Dhabi |
ZIP/Postal code |
Zone 1 |
Country |
United Arab Emirates |
|
|
Platform ID |
GPL26511 |
Series (1) |
GSE226084 |
Microvascular and Macrovascuar Complications of Type 2 Diabetes Mellitus: Exome Wide Association Analyses |
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