|
| Status |
Public on Mar 31, 2024 |
| Title |
MIR100HG knocking down A549 cells 1 |
| Sample type |
SRA |
| |
|
| Source name |
lung
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: lung
cell line: A549
cell type: lung cancer
genotype: MIR100HG knocking down
|
| Treatment protocol |
A549 cells were transiently transfected with ASO-h-MIR100HG_003 or ASO Negative Control #1.
|
| Growth protocol |
A549 cells were maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum, 100 U/ml streptomycin and 100 U/ml penicillin and incubated in 5% CO2 incubator at 37°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from A549 cells using TRNzol Universal total RNA extraction kit.
The library was constructed using Tn5 DNA Library Prep Kit for Illumina according to manufacturers’ instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Adapter sequences were removed from the original sequencing data and low-quality sequences were filtered
Clean data were aligned to reference genome by Hisat 2 software and transcripts were assembled by Stringtie software.
DEseq2 and EdgeR analysis were used to screen out differentially expressed genes.
Assembly: hg19
Supplementary files format and content: tab-delimited text files include RPKM values for each Sample
|
| |
|
| Submission date |
Mar 02, 2023 |
| Last update date |
Mar 31, 2024 |
| Contact name |
Hao Zhang |
| E-mail(s) |
dy_zhanghao@126.com
|
| Phone |
+86-15181023824
|
| Organization name |
Deyang People’s Hospital
|
| Department |
Department of Reproductive and Genetic Diseases
|
| Street address |
No. 173 Taishan North Road
|
| City |
Deyang |
| State/province |
Sichuan |
| ZIP/Postal code |
618000 |
| Country |
China |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE226491 |
MIR100HG promoted ferroptosis via regulating the expression of ACSL3 in lung cancer cells |
|
| Relations |
| BioSample |
SAMN33567252 |
| SRA |
SRX19549241 |
