|
| Status |
Public on Sep 25, 2023 |
| Title |
RC17 master cell bank four passages after thawing |
| Sample type |
genomic |
| |
|
| Source name |
embryonic stem cells
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
|
| Treatment protocol |
n/a
|
| Growth protocol |
Cell lines were grown according to standard culturing conditions, and as described in the relative manuscript
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using QIAGEN Dneasy mini kit according to manufacturer's instruction.
|
| Label |
C-Bio and A-DNP
|
| Label protocol |
C-Bio and A-DPN
|
| |
|
| Hybridization protocol |
As per standard Illumina protocol
|
| Scan protocol |
The BeadChip array was scanned with the iScan (Illumina)
|
| Description |
Genomic DNA extracted from human human embryonic stem cells was genotyped using Human CytoSNP-12 v2.1 BeadChip (Illumina).
|
| Data processing |
The scanned data files were processed by GenomeStudio GT module (Illumina). BluFuse Multi (Illumina) was used to call genotype and calculate LogR ratio and B allele frequency using a manifest file (HumanCytoSNP-12v2-1_L1.bpm) and a cluster file (HumanCytoSNP-12v2-1_L (1).egt) supplied by Illumina.
Genotype: AA,AB,BB,or NC (no call)
|
| |
|
| Submission date |
Apr 14, 2023 |
| Last update date |
Sep 25, 2023 |
| Contact name |
Malin Parmar |
| E-mail(s) |
malin.parmar@med.lu.se
|
| Organization name |
Wallenberg Neuroscience Center, MultiPark and Lund Stem Cell Centre
|
| Department |
Department of Experimental Medical Science, Lund University
|
| Street address |
221 84 Lund
|
| City |
Lund |
| ZIP/Postal code |
Box 117, SE-221 00 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL13829 |
| Series (2) |
| GSE229768 |
Preclinical quality, safety and efficacy of a human embryonic stem cell-derived product for treatment of people with moderate Parkinson’s Disease (STEM-PD) III |
| GSE229769 |
Preclinical quality, safety and efficacy of a human embryonic stem cell-derived product for treatment of people with moderate Parkinson’s Disease (STEM-PD) |
|
