|
| Status |
Public on May 12, 2011 |
| Title |
Digital Genomic Footprinting assay of Fetal Brain; DGF.DS11872 |
| Sample type |
SRA |
| |
|
| Source name |
Fetal Brain primary tissue, day 122; DGF.DS11872
|
| Organism |
Homo sapiens |
| Characteristics |
sample alias: fBrain.H-22510d122
sample common name: Fetal Brain
molecule: genomic DNA
disease: None
biomaterial_provider: University of Washington, Congenital Defects Lab. Ian Glass
biomaterial_type: Primary Tissue
tissue_type: fetal brain
tissue_depot: UW
collection_method: fetal
donor_id: H-22510
donor_age: 122 days
donor_health_status: NA
donor_sex: Male
donor_ethnicity: NA
experiment_type: Digital Genomic Footprinting
extraction_protocol: Qiagen minElut
dnase_protocol: Stamlab DNase Protocol, Sabo, P. J. et al. Nat Methods 3, 511-518 (2006)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Library construction protocol: Single read - Illumina
|
| |
|
| Library strategy |
DNase-Hypersensitivity |
| Library source |
genomic |
| Library selection |
DNAse |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Description |
sample_term_id: UBERON_0000955
assay_term_id: OBI_0001853
nucleic_acid_term_id: SO_0000352
Design description: Digital Genomic Footprinting determination via DNase-seq
Library name: DGF.DS11872
EDACC Genboree Experiment Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FUniversity%20of%20Washington%2FEXPERIMENT%2FEDACC.7450
EDACC Genboree Sample Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FUniversity%20of%20Washington%2FSAMPLE%2FEDACC.2448
****************
For data usage terms and conditions, please refer to:
http://www.drugabuse.gov/funding/funding-opportunities/nih-common-fund/epigenomics-data-access-policies
****************
|
| Data processing |
**********************************************************************
ANALYSIS FILE NAME: GSM723021_UW.Fetal_Brain.Digital_Genomic_Footprinting.H-22510.DGF.DS11872.bed
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: DGF.DS11872.hg19.level.1.release.4
ANALYSIS TITLE: Mapping of Fetal Brain Digital Genomic Footprinting Data
ANALYSIS DESCRIPTION: Illumina reads produced by Digital Genomic Footprinting on Fetal Brain, Donor H-22510, Library DGF.DS11872 were mapped to the human genome using Pash.
ANALYSIS TYPE: REFERENCE_ALIGNMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.7629
DATA_ANALYSIS_LEVEL: 1
EXPERIMENT_TYPE: Digital Genomic Footprinting
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: Pash
SOFTWARE_VERSION: 3.0
MAXIMUM_ALIGNMENT_LENGTH: Read length
MISMATCHES_ALLOWED: 10% of read length
ALIGNMENTS_ALLOWED: 1
TREATMENT_OF_MULTIPLE_ALIGNMENTS: If a read maps to more than 1 position it is removed from consideration.
TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: None
ALIGNMENT_POSTPROCESSING: None
RELEASE_NUMBER: Human Epigenome Atlas 4
QUALITY SCORES:
NUMBER_OF_MAPPED_READS: 219,468,287
NUMBER_OF_DIGITAL_GENOMIC_FOOTPRINTING_EXPERIMENTS_SCORED_IN_THIS_RELEASE: 5
FINDPEAKS_SCORE: 0.6965
FINDPEAKS_PERCENTILE: 100
HOTSPOT_SCORE: 0.6871
HOTSPOT_PERCENTILE: 100
IROC_SCORE: 0.9973
IROC_PERCENTILE: 40
POISSON_SCORE: 0.6608
POISSON_PERCENTILE: 80
**********************************************************************
ANALYSIS FILE NAME: GSM723021_UW.Fetal_Brain.Digital_Genomic_Footprinting.H-22510.DGF.DS11872.wig
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: DGF.DS11872.hg19.level.2.release.4
ANALYSIS TITLE: Raw Signal Density Graphs of Fetal Brain Digital Genomic Footprinting Data
ANALYSIS DESCRIPTION: Illumina Digital Genomic Footprinting read mappings from Fetal Brain, Donor H-22510, Library DGF.DS11872 were processed into density graphs of raw signal representing the aligned read density.
ANALYSIS TYPE: ABUNDANCE_MEASUREMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.7634
DATA_ANALYSIS_LEVEL: 2
EXPERIMENT_TYPE: Digital Genomic Footprinting
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: In house programs and scripts
SOFTWARE_VERSION: NA
READ_EXTENSION: 0bp
GENOMIC_WINDOW: 150bp
TREATMENT_OF_REGIONS_PRONE_TO_MULTIPLE_ALIGNMENTS: None
RELEASE_NUMBER: Human Epigenome Atlas 4
BROWSER_TRACK_NAME: FB DGF 10 72
BROWSER_TRACK_DESCRIPTION: UW Fetal Brain Digital Genomic Footprinting Donor H-22510 Library DGF.DS11872 EA Release 4
QUALITY SCORES:
NUMBER_OF_MAPPED_READS: 219,468,287
NUMBER_OF_DIGITAL_GENOMIC_FOOTPRINTING_EXPERIMENTS_SCORED_IN_THIS_RELEASE: 5
FINDPEAKS_SCORE: 0.6965
FINDPEAKS_PERCENTILE: 100
HOTSPOT_SCORE: 0.6871
HOTSPOT_PERCENTILE: 100
IROC_SCORE: 0.9973
IROC_PERCENTILE: 40
POISSON_SCORE: 0.6608
POISSON_PERCENTILE: 80
**********************************************************************
|
| |
|
| Submission date |
May 11, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Northwest REMC |
| E-mail(s) |
rharris1@bcm.tmc.edu
|
| Organization name |
University of Washington
|
| Street address |
-
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195 |
| Country |
USA |
| |
|
| Platform ID |
GPL10999 |
| Series (2) |
| GSE18927 |
University of Washington Human Reference Epigenome Mapping Project |
| GSE51341 |
Conservation of mouse-human trans-regulatory circuitry despite high cis-regulatory divergence |
|
| Relations |
| SRA |
SRX062364 |
| BioSample |
SAMN00113444 |
| Named Annotation |
GSM723021_UW.Fetal_Brain.Digital_Genomic_Footprinting.H-22510.DGF.DS11872.wig.gz |
