|
| Status |
Public on Dec 29, 2023 |
| Title |
ms477 |
| Sample type |
protein |
| |
|
| Source name |
Serum/plasma
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Serum/plasma
condition: BMchim.564
|
| Treatment protocol |
Retrogenic chimeras were made by reconsitution of irradiated mice with RAG bone marrow transduced with candidate TCRs
|
| Extracted molecule |
protein |
| Extraction protocol |
Blood was collected from mice. The blood was then processed into serum or plasma which was then run on our arrays.
|
| Label |
R-PE conjugated goat anti-mouse IgG secondary antibody
|
| Label protocol |
A secondary antibody conjugated to a fluorophore was added to a PBS solution containing conjugated beads bound to antibodies from samples.
|
| |
|
| Hybridization protocol |
A secondary antibody conjugated to a fluorophore was added to a PBS solution containing conjugated beads bound to antibodies from samples.
|
| Scan protocol |
A Luminex FlexMAP 3D instrument was used to measure fluorescence (in MFI) from each barcoded bead.
|
| Description |
Antibodies in samples bind to conjugated beads in array. The fluorescence measured as MFI from the secondary antibody was used to determine antibody reactivity.
|
| Data processing |
Sample replicate MFI values were averaged for each antigen.
Raw values were used without normalization.
|
| |
|
| Submission date |
May 05, 2023 |
| Last update date |
Dec 29, 2023 |
| Contact name |
Elliot Akama-Garren |
| Organization name |
Harvard Medical School
|
| Street address |
200 Longwood Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL33382 |
| Series (2) |
| GSE231798 |
Loss of B cell Tolerance is TCR Dependent [IgG CTD array] |
| GSE231808 |
Loss of B cell Tolerance is TCR Dependent |
|
