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Sample GSM733674 Query DataSets for GSM733674
Status Public on Jun 02, 2011
Title Bernstein_NHEK_H3K27ac
Sample type SRA
 
Source name NHEK
Organism Homo sapiens
Characteristics donor_id: ENCDO268AAA,ENCDO303AAA
biomaterial_type: primary cell
cell_type: keratinocyte
lab: Broad
lab description: Bernstein - Broad Institute
datatype: ChipSeq
datatype description: Chromatin IP Sequencing
cell organism: human
cell description: epidermal keratinocytes
cell sex: U
antibody antibodydescription: rabbit polyclonal. Antibody Target: H3K27ac
antibody: H3K27ac
antibody targetdescription: Histone H3 (acetyl K27). As with H3K9ac, associated with transcriptional initiation and open chromatin structure. It remains unknown whether acetylation has can have different consequences depending on the specific lysine residue targeted. In general, though, there appears to be high redundancy. Histone acetylation is notable for susceptibility to small molecules and drugs that target histone deacetylases.
antibody vendorname: abcam
antibody vendorid: ab4729
treatment: None
treatment description: No special treatment or protocol applies
control: std
control description: Standard input signal for most experiments.
controlid: wgEncodeEH000072
replicate: 1,3,2
softwareversion: ScriptureVPaperR3
Biomaterial provider Lonza
Growth protocol Keratinocyte_protocol.pdf
Extracted molecule genomic DNA
Extraction protocol For extraction protocol details see: http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeBroadHistone
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer IIx
 
Data processing For data processing details see: http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeBroadHistone
 
Submission date May 27, 2011
Last update date May 15, 2019
Contact name ENCODE DCC
E-mail(s) encode-help@lists.stanford.edu
Organization name ENCODE DCC
Street address 300 Pasteur Dr
City Stanford
State/province CA
ZIP/Postal code 94305-5120
Country USA
 
Platform ID GPL10999
Series (2)
GSE29611 Histone Modifications by ChIP-seq from ENCODE/Broad Institute
GSE51334 DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions
Relations
Reanalyzed by GSM936087
SRA SRX067425
BioSample SAMN00622095
Named Annotation GSM733674_hg19_wgEncodeBroadHistoneNhekH3k27acStdSig.bigWig

Supplementary file Size Download File type/resource
GSM733674_hg19_wgEncodeBroadHistoneNhekH3k27acStdAlnRep1.bam 695.7 Mb (ftp)(http) BAM
GSM733674_hg19_wgEncodeBroadHistoneNhekH3k27acStdAlnRep2.bam 340.5 Mb (ftp)(http) BAM
GSM733674_hg19_wgEncodeBroadHistoneNhekH3k27acStdAlnRep3.bam 157.9 Mb (ftp)(http) BAM
GSM733674_hg19_wgEncodeBroadHistoneNhekH3k27acStdPk.broadPeak.gz 1.0 Mb (ftp)(http) BROADPEAK
GSM733674_hg19_wgEncodeBroadHistoneNhekH3k27acStdSig.bigWig 279.6 Mb (ftp)(http) BIGWIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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