|
| Status |
Public on Jun 02, 2011 |
| Title |
Bernstein_NHEK_H3K36me3 |
| Sample type |
SRA |
| |
|
| Source name |
NHEK
|
| Organism |
Homo sapiens |
| Characteristics |
donor_id: ENCDO268AAA,ENCDO303AAA
biomaterial_type: primary cell
cell_type: keratinocyte
lab: Broad
lab description: Bernstein - Broad Institute
datatype: ChipSeq
datatype description: Chromatin IP Sequencing
cell organism: human
cell description: epidermal keratinocytes
cell sex: U
antibody antibodydescription: rabbit polyclonal. Antibody Target: H3K36me3
antibody: H3K36me3
antibody targetdescription: Specific for histone H3 tri methylated at lysine 36, weakly reacts with H3K36me2. Marks regions of RNAPII elongation, including coding and non-coding transcripts.
antibody vendorname: abcam
antibody vendorid: ab9050
treatment: None
treatment description: No special treatment or protocol applies
control: std
control description: Standard input signal for most experiments.
controlid: wgEncodeEH000072
replicate: 1,3,2
softwareversion: ScriptureVPaperR3
|
| Biomaterial provider |
Lonza
|
| Growth protocol |
Keratinocyte_protocol.pdf
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
For extraction protocol details see: http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeBroadHistone
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Data processing |
For data processing details see: http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeBroadHistone
|
| |
|
| Submission date |
May 27, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
ENCODE DCC |
| E-mail(s) |
encode-help@lists.stanford.edu
|
| Organization name |
ENCODE DCC
|
| Street address |
300 Pasteur Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5120 |
| Country |
USA |
| |
|
| Platform ID |
GPL10999 |
| Series (2) |
| GSE29611 |
Histone Modifications by ChIP-seq from ENCODE/Broad Institute |
| GSE51334 |
DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions |
|
| Relations |
| Reanalyzed by |
GSM936087 |
| SRA |
SRX067477 |
| BioSample |
SAMN00622147 |
| Named Annotation |
GSM733726_hg19_wgEncodeBroadHistoneNhekH3k36me3StdSig.bigWig |
