|
| Status |
Public on May 02, 2012 |
| Title |
HMDM+LPS rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
HMDM
|
| Organism |
Homo sapiens |
| Characteristics |
targets: -
stimulus: LPS
|
| Treatment protocol |
Apoptosis of lymphocytes was induced by g-irradiation (10Gy). HMDM were stimulated with 10 ng/ml ultra-pure LPS.
|
| Growth protocol |
Lymphocytes were maintained for 7 days in RPMI1640, 10% FCS, 2 mM L-Glutamine and 1% penicillin/streptomycin (complete media) containing 50 U/ml rhIL-2. HMDM were generated from human peripheral monocytes, isolated by counter flow elutriation, by culture for 8 days in complete media containing 25 ng/ml rhM-CSF
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted using the Illustra RNAspin Mini Isolation Kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (performed by the personnel of the microarray facility at University of California Irvine)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Gene 1.0 ST Array. GeneChips were washed and stained in the GeneChip Fluidics Stations 450
|
| Scan protocol |
GeneChips were scanned using the GeneChip 3000 Scanner 7G with Autoloader
|
| Description |
0410F-08_5_(HuGene-1_0-st-v1)
gene expression data from LPS-stimulated HMDM
LPS2
|
| Data processing |
The data were analyzed with JMP genomics (v5.0) using the basic expression workflow and median signal intensity as normalization method.
|
| |
|
| Submission date |
Jun 23, 2011 |
| Last update date |
May 02, 2012 |
| Contact name |
Marie Emeline Benoit |
| E-mail(s) |
mbenoit@uci.edu
|
| Organization name |
UC Irvine
|
| Department |
Molecular Biology and Biochemistry
|
| Lab |
Tenner lab
|
| Street address |
2419 McGaugh Hall
|
| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
92697 |
| Country |
USA |
| |
|
| Platform ID |
GPL10739 |
| Series (1) |
| GSE30177 |
Complement protein C1q modulates macrophage activation and inflammasome activity during the uptake of apoptotic cells |
|
