 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Sep 18, 2023 |
| Title |
Aristolochic Acid -induced Nephropathic (ANN) mice. |
| Sample type |
RNA |
| |
|
| Source name |
aortas
|
| Organism |
Mus musculus |
| Characteristics |
treatment: repeated i.p. Aristolochic Acid (AA, x4)
strain: C57BL6
Sex: female
time: day 21
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted with RNeasy Fibrous Tissue Mini Kit
|
| Label |
SYBR green
|
| Label protocol |
RT: Qiagen RT2 first strand kit, qPCR on QuantStudio12 flex
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
AAN
|
| Data processing |
The normalization and all the data analysis were performed according to the manufacturers instructions using their web-based software: https://geneglobe.qiagen.com/us/analyze
For the normalization, average of a combination of automatically selected housekeeping genes (out of 5 tested Actb,B2m, Gapdh, Gusb, Hsp90ab1) were used
Target gene signals normalized to housekeeping genes; 2^-ΔCt, where ΔCt = (Ct_Target − Ct_HKG)]
The web-based software package automatically performs all deltadeltaCt based fold-change calculations from the uploaded raw threshold cycle data.
Fold-changes are 2^-ΔΔCt, where ΔΔCt = (ΔCt_Test Group - ΔCt_Control group)]
Matrix normalized worksheet reports normalized signal (against housekeeping genes).
Fold Change worksheet reports AAN/PBS or AAN+ Paquinimod/PBS ratios.
|
| |
|
| Submission date |
Jun 29, 2023 |
| Last update date |
Sep 18, 2023 |
| Contact name |
Anne-Catherine Raby |
| E-mail(s) |
rabya@cardiff.ac.uk
|
| Phone |
02920687324
|
| Organization name |
Cardiff University
|
| Street address |
Heath Park, Academic Avenue
|
| City |
Cardiff |
| State/province |
Cardiff |
| ZIP/Postal code |
CF14 4XN |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL29397 |
| Series (2) |
| GSE236194 |
Therapeutic targeting of Chronic Kidney Disease-associated DAMPs differentially contributing to vascular pathology. [atherosclerosis AAN+Paquinimod aortas] |
| GSE236195 |
Therapeutic targeting of Chronic Kidney Disease-associated DAMPs differentially contributing to vascular pathology. |
|
| Supplementary data files not provided |
| Processed data are available on Series record |
|
|
|
|
 |
