|
| Status |
Public on Mar 14, 2012 |
| Title |
CD8 H3K36me3 |
| Sample type |
SRA |
| |
|
| Source name |
Mature CD8+ T cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: Mature CD8+ T cells from thymus
chip antibody: H3K36me3
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Nuclei were treated with Mnase to generation mononucleosomes. These were then immunoprecipitated with rabbit polyclonal antibodies against H3K4me3 and H3K36me3. DNA fragements were eluted and adaptors ligated to allow for ultra high throughput sequencing
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Description |
Chromatin IP against H3K36me3
|
| Data processing |
Alignment: Sequence reads were obtained and mapped to the mouse genome (mm9 assembly) using the Illumina Illumina ELAND software.
|
| |
|
| Submission date |
Jul 12, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Mark Chong |
| E-mail(s) |
mchong@svi.edu.au
|
| Phone |
61-3-92313444
|
| Organization name |
St Vincent's Institute of Medical Research
|
| Street address |
9 Princes Street
|
| City |
Fitzroy |
| State/province |
Victoria |
| ZIP/Postal code |
3065 |
| Country |
Australia |
| |
|
| Platform ID |
GPL9185 |
| Series (2) |
| GSE30580 |
Chromatin maps of mature T cells |
| GSE30584 |
RNA mapping of Drosha deficient cells, chromatin maps of mature T cells, and miRNA profiling of lymphopoiesis |
|
| Relations |
| SRA |
SRX083256 |
| BioSample |
SAMN00668295 |
