|
| Status |
Public on Mar 14, 2012 |
| Title |
CD8SP_miRNAseq |
| Sample type |
SRA |
| |
|
| Source name |
CD8SP cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
source tissue: Thymus
cell population: CD4- CD8+ CD3hi CD90+ CD24lo
|
| Extracted molecule |
total RNA |
| Extraction protocol |
19-24nt small RNAs fractionated by gel electrophoresis. Adaptors were ligated only to species with 5' phosphate and 3' hydroxyl
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Data processing |
Reads were first clipped of any adaptor sequences (because miRNAs are shorter than the 36bp reads of the GAII platform). The processed reads were then aligned to mature microRNA sequences as annotated in miRBase. Sequences not mapping to mature microRNAs were then mapped to pre-miRNA sequences as annotated in miRBase.
|
| |
|
| Submission date |
Jul 12, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Mark Chong |
| E-mail(s) |
mchong@svi.edu.au
|
| Phone |
61-3-92313444
|
| Organization name |
St Vincent's Institute of Medical Research
|
| Street address |
9 Princes Street
|
| City |
Fitzroy |
| State/province |
Victoria |
| ZIP/Postal code |
3065 |
| Country |
Australia |
| |
|
| Platform ID |
GPL9250 |
| Series (2) |
| GSE30581 |
MicroRNA profiling of murine T lymphopoiesis |
| GSE30584 |
RNA mapping of Drosha deficient cells, chromatin maps of mature T cells, and miRNA profiling of lymphopoiesis |
|
| Relations |
| SRA |
SRX083241 |
| BioSample |
SAMN00668280 |
