|
| Status |
Public on Dec 11, 2023 |
| Title |
RPE-19 cells treated with 0.1 µg/mL GPE and stressed with 500 µM H2O2 32 |
| Sample type |
RNA |
| |
|
| Source name |
Human retinal pigment epithelium (RPR-19) cell, CRL-2502
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Spontaneously immortalized cell line
activation agent: Gac peel extract
treatment time: 72 h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol™ Reagent (Life Technologies, Melbourne, Australia) as per the manufacturer’s protocol
|
| Label |
SYBR Green
|
| Label protocol |
The qPCR was performed on a Qiagen Rotor-Gene Q (Qiagen, Germany) and analysed with inbuilt software (version 2.3.1.49). The thermal cycling conditions were as follows: initial activation at 95°C for 2 min, 45 cycles of 95°C 5 s, 60°C for 10 s, 72°C for 15 s, and melting curve from 65°C to 95°C
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
Test
|
| Data processing |
The normalization and all the data analysis were performed according to the manufacturers instructions using their web-based software package: https://rotor-gene-q.software.informer.com/2.3/
For the normalization it uses housekeeping gene: GAPDH.
Target gene signals normalized to housekeeping genes; 2^-deltaCt, where deltaCt = (Ct_Target − Ct_HKG)]
|
| |
|
| Submission date |
Jul 20, 2023 |
| Last update date |
Dec 11, 2023 |
| Contact name |
Dao Nguyen |
| E-mail(s) |
daonguyen24@gmail.com
|
| Phone |
0410378490
|
| Organization name |
Royal Melbourne Institute of Technology
|
| Department |
School of Science and Health and Biomedical Science
|
| Lab |
Plant
|
| Street address |
289 McKimmies Rd
|
| City |
Bundoora |
| State/province |
Victoria |
| ZIP/Postal code |
3083 |
| Country |
Australia |
| |
|
| Platform ID |
GPL33598 |
| Series (1) |
| GSE237892 |
Real-time quantitative PCR analysis of Nrf2, HO-1, IL-6 and VEGF-A genes in human retinal pigment epithelium (RPE-19) cells |
|
