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Status |
Public on Dec 11, 2023 |
Title |
RPE-19 cells treated with 1 µg/mL GPE and stressed with 500 µM H2O2 33 |
Sample type |
RNA |
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Source name |
Human retinal pigment epithelium (RPR-19) cell, CRL-2502
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Organism |
Homo sapiens |
Characteristics |
cell type: Spontaneously immortalized cell line activation agent: Gac peel extract treatment time: 72 h
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using TRIzol™ Reagent (Life Technologies, Melbourne, Australia) as per the manufacturer’s protocol
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Label |
SYBR Green
|
Label protocol |
The qPCR was performed on a Qiagen Rotor-Gene Q (Qiagen, Germany) and analysed with inbuilt software (version 2.3.1.49). The thermal cycling conditions were as follows: initial activation at 95°C for 2 min, 45 cycles of 95°C 5 s, 60°C for 10 s, 72°C for 15 s, and melting curve from 65°C to 95°C
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Hybridization protocol |
n/a
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Scan protocol |
n/a
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Description |
Test
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Data processing |
The normalization and all the data analysis were performed according to the manufacturers instructions using their web-based software package: https://rotor-gene-q.software.informer.com/2.3/ For the normalization it uses housekeeping gene: GAPDH. Target gene signals normalized to housekeeping genes; 2^-deltaCt, where deltaCt = (Ct_Target − Ct_HKG)]
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Submission date |
Jul 20, 2023 |
Last update date |
Dec 11, 2023 |
Contact name |
Dao Nguyen |
E-mail(s) |
daonguyen24@gmail.com
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Phone |
0410378490
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Organization name |
Royal Melbourne Institute of Technology
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Department |
School of Science and Health and Biomedical Science
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Lab |
Plant
|
Street address |
289 McKimmies Rd
|
City |
Bundoora |
State/province |
Victoria |
ZIP/Postal code |
3083 |
Country |
Australia |
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|
Platform ID |
GPL33598 |
Series (1) |
GSE237892 |
Real-time quantitative PCR analysis of Nrf2, HO-1, IL-6 and VEGF-A genes in human retinal pigment epithelium (RPE-19) cells |
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