|
| Status |
Public on Sep 01, 2011 |
| Title |
iPSC-C-G4 |
| Sample type |
genomic |
| |
|
| Source name |
iPSC line G4
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Human induced pluripotent stem cells (hIPSCs)
genetic modification: Reprogramming and gene targeting
|
| Treatment protocol |
n/a
|
| Growth protocol |
Cell lines were grown according to standard conditions, and as described in associated manuscript
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using DNeasy kit (Qiagen) according to the manufacturer's instruction.
|
| Label |
C-Bio and A-DNP
|
| Label protocol |
As per standard Illumina protocol.
|
| |
|
| Hybridization protocol |
DNA was amplified, fragmented and hybridized to each BeadChip arrays according to the manufacturer's instructions.
|
| Scan protocol |
The BeadChip arrays were scanned using the iScan (Illumina)
|
| Description |
Genomic DNA extracted from iPSCs was genotyped using Illumina CytoSNP12 v2.1 BeadChips.
|
| Data processing |
The scanned data files were processed by GenomeStudio GT module (Illumina). GenomeStudio was also used to call genotype and calculate LogR ratio and B allele frequency using a cluster file (HumanCytoSNP-12v2-1_A.egt) supplied by Illumina.
|
| |
|
| Submission date |
Jul 29, 2011 |
| Last update date |
Sep 01, 2011 |
| Contact name |
Kosuke Yusa |
| Organization name |
Wellcome Trust Sanger Institute
|
| Street address |
Wellcome Trust Genome Campus
|
| City |
Hinxton |
| State/province |
Cambridgeshire |
| ZIP/Postal code |
CB10 1SA |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL13829 |
| Series (1) |
| GSE31035 |
SNP analysis of human induced pluripotent stem cells |
|
