|
Status |
Public on Dec 28, 2011 |
Title |
ChIP-Seq analysis of WCE in human ADMSC Diff Day20 cells; Lib EpiLabSPL Row 1757 |
Sample type |
SRA |
|
|
Source name |
Adipose-derived mesenchymal stem cells were cultured in adipocyte differentiation medium for 20 days; Lib EpiLabSPL Row 1757
|
Organism |
Homo sapiens |
Characteristics |
sample alias: 213A sample common name: Mesenchymal Stem Cell Derived Adipocyte Cultured Cells donor_health_status: NA molecule: genomic DNA disease: none markers: NA culture_conditions: Differentiation Method: Mesenchymal stem cells were differentiated into adipocytes by using 3-isobutyl-1-methyl xanthine (IBMX), rosiglitazone, insulin, and dexamethasone passage_if_expanded: NA cell_type: ADMSC Diff Day20 donor_ethnicity: NA donor_sex: Female biomaterial_type: Primary Cell Culture donor_id: 93 biomaterial_provider: Harvard University donor_age: 41 extraction_protocol_sonication_cycles: 1 chip_protocol_chromatin_amount: standard extraction_protocol: SDS lysis experiment_type: ChIP-Seq Input chip_protocol: Input extraction_protocol_type_of_sonicator: Bioruptor
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Library construction protocol: Single read - Illumina
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|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
RANDOM |
Instrument model |
Illumina Genome Analyzer IIx |
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|
Description |
sample_term_id: CL_0000136 assay_term_id: OBI_0000716 nucleic_acid_term_id: SO_0000352 Design description: Human Chromatin IP REMC Sequencing on Illumina Library name: Lib EpiLabSPL Row 1757 EDACC Genboree Experiment Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FEXPERIMENT%2FEDACC.8001 EDACC Genboree Sample Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FSAMPLE%2FEDACC.8182 **************** For data usage terms and conditions, please refer to: http://www.drugabuse.gov/funding/funding-opportunities/nih-common-fund/epigenomics-data-access-policies ****************
|
Data processing |
**********************************************************************
ANALYSIS FILE NAME: GSM772740_BI.Mesenchymal_Stem_Cell_Derived_Adipocyte_Cultured_Cells.Input.93.bed ANALYSIS CENTER: EDACC ANALYSIS ALIAS: Lib EpiLabSPL Row 1757.hg19.level.1.release.5 ANALYSIS TITLE: Mapping of Mesenchymal Stem Cell Derived Adipocyte Cultured Cells ChIP-Seq Input Data ANALYSIS DESCRIPTION: Illumina reads produced by ChIP-Seq Input on Mesenchymal Stem Cell Derived Adipocyte Cultured Cells, Donor 93, were mapped to the human genome using Pash. ANALYSIS TYPE: REFERENCE_ALIGNMENT EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.10743 DATA_ANALYSIS_LEVEL: 1 EXPERIMENT_TYPE: ChIP-Seq GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19 SOFTWARE: Pash SOFTWARE_VERSION: 3.0 MAXIMUM_ALIGNMENT_LENGTH: Read length MISMATCHES_ALLOWED: 10% of read length ALIGNMENTS_ALLOWED: 1 TREATMENT_OF_MULTIPLE_ALIGNMENTS: If a read maps to more than 1 position it is removed from consideration. TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: If multiple reads map to the same start position on the + strand or stop position on the - strand, only a single read is retained. ALIGNMENT_POSTPROCESSING: None READ_EXTENSION: 200bp RELEASE_NUMBER: Human Epigenome Atlas 5
QUALITY SCORES: NUMBER_OF_MAPPED_READS: 36,969,381 NUMBER_OF_Input_EXPERIMENTS_SCORED_IN_THIS_RELEASE: 56 FINDPEAKS_SCORE: 0.0047 FINDPEAKS_PERCENTILE: 34 HOTSPOT_SCORE: 0.0044 HOTSPOT_PERCENTILE: 23 IROC_SCORE: 1.0 IROC_PERCENTILE: 100 POISSON_SCORE: 0.0324 POISSON_PERCENTILE: 21 MAXIMUM_REPLICATE_CORRELATION: 0.95
**********************************************************************
ANALYSIS FILE NAME: GSM772740_BI.Mesenchymal_Stem_Cell_Derived_Adipocyte_Cultured_Cells.Input.93.wig ANALYSIS CENTER: EDACC ANALYSIS ALIAS: Lib EpiLabSPL Row 1757.hg19.level.2.release.5 ANALYSIS TITLE: Raw Signal Density Graphs of Mesenchymal Stem Cell Derived Adipocyte Cultured Cells ChIP-Seq Input Data ANALYSIS DESCRIPTION: Illumina ChIP-Seq Input read mappings from Mesenchymal Stem Cell Derived Adipocyte Cultured Cells, Donor 93, were processed into density graphs of raw signal representing the aligned read density. ANALYSIS TYPE: ABUNDANCE_MEASUREMENT EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.11007 DATA_ANALYSIS_LEVEL: 2 EXPERIMENT_TYPE: ChIP-Seq GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19 SOFTWARE: In house programs and scripts SOFTWARE_VERSION: NA READ_EXTENSION: 200bp TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: If multiple reads map to the same start position on the + strand or stop position on the - strand, only a single read is retained. GENOMIC_WINDOW: 20bp TREATMENT_OF_REGIONS_PRONE_TO_MULTIPLE_ALIGNMENTS: None RELEASE_NUMBER: Human Epigenome Atlas 5 BROWSER_TRACK_NAME: MSCDA Input 93 57 BROWSER_TRACK_DESCRIPTION: BI Mesenchymal Stem Cell Derived Adipocyte Cultured Cells ChIP-Seq Input Donor 93 Library Lib EpiLabSPL Row 1757 EA Release 5
QUALITY SCORES: NUMBER_OF_MAPPED_READS: 36,969,381 NUMBER_OF_Input_EXPERIMENTS_SCORED_IN_THIS_RELEASE: 56 FINDPEAKS_SCORE: 0.0047 FINDPEAKS_PERCENTILE: 34 HOTSPOT_SCORE: 0.0044 HOTSPOT_PERCENTILE: 23 IROC_SCORE: 1.0 IROC_PERCENTILE: 100 POISSON_SCORE: 0.0324 POISSON_PERCENTILE: 21 MAXIMUM_REPLICATE_CORRELATION: 0.95
**********************************************************************
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|
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Submission date |
Aug 03, 2011 |
Last update date |
Jan 29, 2015 |
Contact name |
BROAD INSTITUTE |
E-mail(s) |
rharris1@bcm.tmc.edu
|
Organization name |
Broad Institute
|
Street address |
-
|
City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02142 |
Country |
USA |
|
|
Platform ID |
GPL10999 |
Series (1) |
GSE17312 |
BI Human Reference Epigenome Mapping Project |
|
Relations |
BioSample |
SAMN00691450 |
Named Annotation |
GSM772740_BI.Mesenchymal_Stem_Cell_Derived_Adipocyte_Cultured_Cells.Input.93.wig.gz |