|
| Status |
Public on Jan 15, 2014 |
| Title |
ChIP-Seq analysis of H3K4me3 in human CD15 cells; Lib EpiLabSPL Row 1797 |
| Sample type |
SRA |
| |
|
| Source name |
flow sorted CD15+ cells from cord blood; Lib EpiLabSPL Row 1797
|
| Organism |
Homo sapiens |
| Characteristics |
sample alias: 28.0
sample common name: CD15 Primary Cells
molecule: genomic DNA
disease: none
biomaterial_provider: FHCRC
biomaterial_type: Primary Cell
cell_type: CD15 Primary Cells
markers: CD15+
donor_id: 23.0
donor_age: NA
donor_health_status: none
donor_sex: Male
donor_ethnicity: NA
passage_if_expanded: NA
chip_protocol_bead_type: Protein A-Sepharose 4B
chip_antibody: H3K4me3
chip_protocol_chromatin_amount: 10 million cells
extraction_protocol: SDS lysis
chip_protocol_antibody_amount: 2-15 ug
chip_protocol_bead_amount: 70 ul bed volume
chip_protocol: Bernstein_BROAD_ENCODE_protocol
extraction_protocol_sonication_cycles: 1
chip_antibody_catalog: 07-473
chip_antibody_provider: Millipore
chip_antibody_lot: DAM1623866
experiment_type: Histone H3K4me3
extraction_protocol_type_of_sonicator: Bioruptor
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Library construction protocol: Single read - Illumina
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Description |
sample_term_id: CL_0000775
assay_term_id: OBI_0000716
nucleic_acid_term_id: SO_0000352
Design description: Human Chromatin IP REMC Sequencing on Illumina
Library name: Lib EpiLabSPL Row 1797
EDACC Genboree Experiment Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FEXPERIMENT%2FEDACC.8043
EDACC Genboree Sample Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FSAMPLE%2FEDACC.2562
****************
For data usage terms and conditions, please refer to:
http://www.drugabuse.gov/funding/funding-opportunities/nih-common-fund/epigenomics-data-access-policies
****************
|
| Data processing |
Various levels of processed data files will be made available as this project proceeds.
|
| |
|
| Submission date |
Aug 03, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
BROAD INSTITUTE |
| E-mail(s) |
rharris1@bcm.tmc.edu
|
| Organization name |
Broad Institute
|
| Street address |
-
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL10999 |
| Series (1) |
| GSE17312 |
BI Human Reference Epigenome Mapping Project |
|
| Relations |
| SRA |
SRX088697 |
| BioSample |
SAMN00012132 |
