|
| Status |
Public on Sep 04, 2023 |
| Title |
DSHN-4-D4_08 |
| Sample type |
RNA |
| |
|
| Source name |
Dendritic cells isolated from lungs
|
| Organism |
Mus musculus |
| Characteristics |
cancer cell grafted: 4T1 grafted
treatment: DSHN
dosage: 10mg/kg
treatment frequency: Daily for 7 days
|
| Treatment protocol |
24 mice were separated evenly into two treatment groups (placebo vs. DSHN). 10 days post-grafting was allowed for metastasis to occur before treatments. Dosage was 10mg/kg/mouse, and mice were treated daily for 7 days. Lungs were collected and 3 lungs from the same treatment group were pooled to be one sample.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Pooled lung samples were processed immediately for dissociation using Lung Dissociation Kit from Miltenyi Biotec (130-095-927). Dendritic cells were isolated from each pooled sample using CD11b (130-126-725) and CD11c (130-125-835) beads from Miltenyi Biotec. RNA was isolated using Qiagen RNeasy Mini Kit
|
| Label |
N/A
|
| Label protocol |
n/a
|
| |
|
| Hybridization protocol |
Hybridization reaction was set up based on the NanoString manuals. We calculated required amounts of RNA samples based on DV200, mix with the NanoString reporter CodeSet, hybridization buffer and Capture ProbeSet. The hybridization master mix was incubated at 65C for 20 hours and then loaded to the cartridge to run the assay.
|
| Scan protocol |
NanoString nCounter Sprint Profiler was used to examine RNA targets in samples; Mouse Myeloid Innate Immunity V2 was selected.
|
| Data processing |
nSolver Analysis Software 4.0 was the software used for data analysis; data was analyzed according to the user manual
|
| |
|
| Submission date |
Aug 30, 2023 |
| Last update date |
Sep 04, 2023 |
| Contact name |
Erik Nelson |
| E-mail(s) |
enels@illinois.edu
|
| Phone |
(217) 244-5477
|
| Organization name |
University of Illinois at Urbana-Champaign
|
| Lab |
Erik Nelson
|
| Street address |
523 Burrill, 407 South Goodwin Ave
|
| City |
Urbana |
| State/province |
IL |
| ZIP/Postal code |
61801 |
| Country |
USA |
| |
|
| Platform ID |
GPL25266 |
| Series (2) |
| GSE241917 |
Re-education of myeloid immune cells to reduce regulatory T cell expansion and impede breast cancer progression [Nanostring] |
| GSE241918 |
Re-education of myeloid immune cells to reduce regulatory T cell expansion and impede breast cancer progression |
|
