|
| Status |
Public on Jan 10, 2024 |
| Title |
TB_23110_2mo-Plasma IgA |
| Sample type |
protein |
| |
|
| Source name |
2mo-Plasma
|
| Organism |
Macaca fascicularis |
| Characteristics |
infection states: Symptomatic uncontrolled Mtb infection/TB disease
|
| Treatment protocol |
BALF (1:35) or plasma (1:100)
|
| Growth protocol |
N/A
|
| Extracted molecule |
protein |
| Extraction protocol |
Plasma was obtained from peripheral blood samples and the BAL procedure was performed as previously described via direct visualization of the airway and lavage of ~ 40 ml normal saline (0.9%). Animal protocols and procedures were approved by the University of Pittsburgh’s Institutional Animal Care and Use Committee before the collection of samples.
|
| Label |
Cy3 Goat Anti-Human Serum IgA
|
| Label protocol |
Cy3 Goat Anti-Human Serum IgA (Jackson ImmunoResearch Labs Cat# 109-165-011, RRID:AB 2337721)
|
| |
|
| Hybridization protocol |
Slides with 16 arrays on each were blocked SuperBlock (Thermo Fisher Scientific), then incubated with BALF (1:35) or plasma (1:100) diluted in phosphate buffered saline with 5% skim milk and 0.2% tween 20 (PBST) for 16 hrs at 4°C. After washing with 5% skim milk and 0.2% tween 20 (PBST) (3 x 5 min), slides were incubated with Cy3 Goat Anti-Human Serum IgA (Jackson ImmunoResearch Labs Cat# 109-165-011, RRID:AB_2337721) and Alexa 647 Goat Anti-Human IgG (Jackson ImmunoResearch Labs Cat# 109-605-008, RRID:AB_2337882; diluted at 1:200 in 5% skim milk and 0.2% tween 20 (PBST)) for 2 hrs at 4°C
|
| Scan protocol |
Slides were scanned at 635 nm and 535 nm with a Tecan PowerScanner, and images were quantified with the ArrayPro Analyzer Software (Media Cybernetics, Inc.).
|
| Description |
IgA
|
| Data processing |
Spots were considered reactive if the ratio of the median fluorescent intensity (MFI) of the gene was greater than 1.4 x the median of negative control genes
|
| |
|
| Submission date |
Sep 20, 2023 |
| Last update date |
Jan 10, 2024 |
| Contact name |
Jacqueline M. Achkar |
| E-mail(s) |
Jacqueline.achkar@einsteinmed.edu
|
| Phone |
7184302971
|
| Organization name |
Albert Einstein College of Medicine
|
| Street address |
1300 Morris Park Ave
|
| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platform ID |
GPL33775 |
| Series (2) |
| GSE243660 |
Mucosal and systemic antigen-specific antibody responses correlate with protection against active tuberculosis in nonhuman primates (NAPPA IgA) |
| GSE243663 |
Mucosal and systemic antigen-specific antibody responses correlate with protection against active tuberculosis in nonhuman primates |
|
