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| Status |
Public on Nov 22, 2023 |
| Title |
S.epidermidis-injected mice |
| Sample type |
RNA |
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| Source name |
aortas
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| Organism |
Mus musculus |
| Characteristics |
Sex: Female
strain: C57BL6
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted with RNeasy Fibrous Tissue Mini Kit
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| Label |
SYBR green
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| Label protocol |
RT: Qiagen RT2 first strand kit, qPCR on QuantStudio12 flex
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| Hybridization protocol |
n/a
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| Scan protocol |
n/a
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| Description |
Live S.epidermidis i.p. administration
AAN
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| Data processing |
The normalization and all the data analysis were performed according to the manufacturers instructions using their web-based software: https://geneglobe.qiagen.com/us/analyze
For the normalization, average of a combination of automatically selected housekeeping genes (out of 5 tested Actb,B2m, Gapdh, Gusb, Hsp90ab1) were used
Target gene signals normalized to housekeeping genes; 2^-ΔCt, where ΔCt = (Ct_Target − Ct_HKG)]
The web-based software package automatically performs all deltadeltaCt based fold-change calculations from the uploaded raw threshold cycle data.
Fold-changes are 2^-ΔΔCt, where ΔΔCt = (ΔCt_Test Group - ΔCt_Control group)]
Matrix normalized worksheet reports normalized signal (against housekeeping genes Actb, B2m, Gusb).
Fold Change worksheet reports S.epidermidis/Control or S.epidermidis + Paquinimod/Control ratios.
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| Submission date |
Oct 24, 2023 |
| Last update date |
Nov 22, 2023 |
| Contact name |
Anne-Catherine Raby |
| E-mail(s) |
rabya@cardiff.ac.uk
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| Phone |
02920687324
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| Organization name |
Cardiff University
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| Street address |
Heath Park, Academic Avenue
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| City |
Cardiff |
| State/province |
Cardiff |
| ZIP/Postal code |
CF14 4XN |
| Country |
United Kingdom |
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| Platform ID |
GPL29397 |
| Series (2) |
| GSE246100 |
Calprotectin blockade inhibits long-term vascular pathology following peritoneal dialysis-associated bacterial infection. [1] |
| GSE246102 |
Calprotectin blockade inhibits long-term vascular pathology following peritoneal dialysis-associated bacterial infection. |
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| Supplementary data files not provided |
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