|
Status |
Public on May 01, 2024 |
Title |
8175_5 |
Sample type |
SRA |
|
|
Source name |
Hepa1-6
|
Organism |
Mus musculus |
Characteristics |
cell line: Hepa1-6 cell type: mouse hepatocarcinoma cells
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Description |
8175_5
|
Data processing |
Illumina Casava1.7 software used for basecalling. Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm8 whole genome using bowtie v0.12.2 with parameters -q -p 4 -e 100 -y -a -m 10 --best --strata Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using a protocol from Chepelev et al., Nucleic Acids Research, 2009. In short, exons from all isoforms of a gene were merged to create one meta-transcript. The number of reads falling in the exons of this meta-transcript were counted and normalized by the size of the meta-transcript and by the size of the library. Assembly: mm8 Supplementary files format and content: tab-delimited text files include RPKM values for each Sample ...
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|
|
Submission date |
Nov 08, 2023 |
Last update date |
May 01, 2024 |
Contact name |
Huichen Song |
E-mail(s) |
DG1830025@smail.nju.edu.cn
|
Phone |
15150516380
|
Organization name |
Nanjing university
|
Street address |
Qixia District, Xianlin Road No.163
|
City |
Nanjing |
State/province |
Jiangsu |
ZIP/Postal code |
210000 |
Country |
China |
|
|
Platform ID |
GPL17021 |
Series (2) |
GSE247265 |
Burdock miR8175 improves insulin sensitivity in DIO mice by targeting PTP1B and PTPRF [I] |
GSE248827 |
Burdock miR8175 improves insulin sensitivity in DIO mice by targeting PTP1B and PTPRF |
|
Relations |
BioSample |
SAMN38154630 |
SRA |
SRX22415698 |